Mugla Sitki Kocman University, Faculty of Science, Chemistry Department, Kotekli, Mugla, Turkey.
Mugla Sitki Kocman University, Faculty of Science, Chemistry Department, Kotekli, Mugla, Turkey.
Mater Sci Eng C Mater Biol Appl. 2019 Aug;101:88-91. doi: 10.1016/j.msec.2019.03.079. Epub 2019 Mar 23.
Cancer Biomarkers are important biological molecules which provide early detection, diagnosis and classification of cancer cells. In this work, nanoparticle tags with different redox potentials have been used as an electrochemical coding technology for the simultaneous detection of carcinoembryonic antigen (CEA), vascular endothelial growth factor (VEGF) and α-fetoprotein (AFP) biomarkers. As far as we know, this is the only work that covers the simultaneous detections of these biomarkers. For this purpose, basically, an electrochemical sandwich immunosensor, which was fabricated from carbon screen printed electrode, was utilized as a diagnostic platform. As nanoparticle tags, PbAu@ɣFeO, CuAu@ɣFeO and ZnAu@ɣFeO hybrid nanolabels were synthesized and used for labelling anti-CEA, anti-VEGF and anti-AFP, respectively. By monitoring differential pulse voltammetric oxidation peaks of labeling metals (Pb, Cu, Zn), CEA, VEGF and AFP biomarkers were detected at the same time.
癌症生物标志物是重要的生物分子，可提供癌细胞的早期检测、诊断和分类。在这项工作中，具有不同氧化还原电位的纳米粒子标签被用作电化学编码技术，用于同时检测癌胚抗原（CEA）、血管内皮生长因子（VEGF）和甲胎蛋白（AFP）生物标志物。据我们所知，这是唯一涵盖这些生物标志物同时检测的工作。为此，基本上利用了由碳丝网印刷电极制成的电化学三明治免疫传感器作为诊断平台。作为纳米粒子标签，分别合成了 PbAu@ɣFeO、CuAu@ɣFeO 和 ZnAu@ɣFeO 杂化纳米标签，用于标记抗 CEA、抗 VEGF 和抗 AFP。通过监测标记金属（Pb、Cu、Zn）的差分脉冲伏安氧化峰，同时检测 CEA、VEGF 和 AFP 生物标志物。
