Piccoli F, Guarneri P, Guarneri R, Ponte F
Neurochem Res. 1986 Dec;11(12):1719-26. doi: 10.1007/BF00967750.
The rat retina and the different brain regions contain membranes sites that bind L-lysine in the nanomolar range. These binding sites undergo changes in different experimental conditions, thus: intraocular injection of kainic acid induces a reduction of the density of L-lysine binding sites, D,L-alpha-aminoadipic acid injected into the eye enhances both kinetic parameters (Bmax and Kd) of L-[3H]lysine binding sites, the intraperitoneal injection of iodoacetic acid decreases the sensitivity for its ligand binding sites, and the exposure to darkness of the rats reduces L-[3H]lysine binding in the retina, thalamus, hypothalamus and superior colliculus, but not in the occipital cortex; such a decrease appears to be characterized, at least in the retina, by a lower sensitivity of the binding sites for L-lysine after the exposure to darkness. The results show that L-lysine binding sites are located on kainic acid-sensitive cells and can be involved in the physiological mechanism of vision.
大鼠视网膜和不同脑区含有能在纳摩尔范围内结合L-赖氨酸的膜位点。这些结合位点在不同实验条件下会发生变化，具体如下：眼内注射 kainic 酸会导致L-赖氨酸结合位点密度降低，向眼内注射D,L-α-氨基己二酸会增强L-[3H]赖氨酸结合位点的两个动力学参数（Bmax和Kd），腹腔注射碘乙酸会降低其配体结合位点的敏感性，大鼠暴露于黑暗环境会降低视网膜、丘脑、下丘脑和上丘中L-[3H]赖氨酸的结合，但枕叶皮质不受影响；这种降低至少在视网膜中表现为暴露于黑暗后L-赖氨酸结合位点的敏感性降低。结果表明，L-赖氨酸结合位点位于对kainic酸敏感的细胞上，可能参与视觉的生理机制。
