Department of Microecology, College of Basic Medical Sciences, Dalian Medical University, Dalian, PR China.
Dalian Medical University, Dalian, PR China.
Nutrition. 2019 Sep;65:50-59. doi: 10.1016/j.nut.2019.03.001. Epub 2019 Mar 11.
Dyslipidemia is an important risk factor for cardiovascular diseases. Fucoidan (FUC) is a polysaccharide extracted from brown marine algae with various biological activities. Galactooligosaccharides (GOS) are important prebiotics that exert benefits on the intestinal microbiota. The aim of this study was to investigate the effects of FUC and GOS on dyslipidemia in rats by modulating the gut microbiota and bile acid metabolism.
Twenty-four male inbred Sprague-Dawley (SD) rats aged 8 wk were fed a normal or high-fat diet (HFD) for 8 wk. During the feeding period, rats were gavaged with normal saline solution, FUC solution (100 mg/kg),or GOS solution (800 mg/kg), or a combination of both once daily. Serum biochemical parameters were determined, and the gut microbiota were analyzed via 16S rRNA gene sequencing. Bile salt hydrolase (BSH) activity in the small intestinal contents was also analyzed. The effects of FUC and GOS on Lactobacillus casei DM8121 were analyzed in vitro.
In rats, GOS and FUC supplementation significantly improved serum total cholesterol, low-density lipoprotein cholesterol, lipopolysaccharide, serum total bile acid, hepatic tissue steatosis, aortic arch injury, gut microbiota, serum high-density lipoprotein cholesterol, cholesterol 7-alpha hydroxylase expression in the liver, and BSH activity in the small intestinal contents. In an in vitro experiment, GOS and FUC supplementation significantly increased L. casei DM8121's BSH activity.
In rats, FUC and GOS supplementation improved serum dyslipidemia, gut microbiota, BSH activity, and bile acid metabolism-related pathways. In vitro, GOS and FUC supplementation increased L. casei DM8121's BSH activity.
血脂异常是心血管疾病的重要危险因素。褐藻糖胶（FUC）是从褐藻中提取的一种多糖，具有多种生物活性。半乳糖低聚糖（GOS）是一种重要的益生元，对肠道微生物群有益。本研究旨在通过调节肠道微生物群和胆汁酸代谢来研究 FUC 和 GOS 对大鼠血脂异常的影响。
将 24 只 8 周龄雄性近交系 Sprague-Dawley（SD）大鼠分别喂食正常或高脂肪饮食（HFD）8 周。在喂养期间，大鼠每天灌胃生理盐水、FUC 溶液（100mg/kg）或 GOS 溶液（800mg/kg）或两者的混合物。测定血清生化参数，通过 16S rRNA 基因测序分析肠道微生物群。还分析了小肠内容物中胆盐水解酶（BSH）的活性。体外分析了 FUC 和 GOS 对干酪乳杆菌 DM8121 的影响。
在大鼠中，GOS 和 FUC 补充显著改善了血清总胆固醇、低密度脂蛋白胆固醇、脂多糖、血清总胆汁酸、肝组织脂肪变性、主动脉弓损伤、肠道微生物群、血清高密度脂蛋白胆固醇、肝脏胆固醇 7-α羟化酶表达和小肠内容物 BSH 活性。在体外实验中，GOS 和 FUC 补充显著增加了干酪乳杆菌 DM8121 的 BSH 活性。
在大鼠中，FUC 和 GOS 补充改善了血清血脂异常、肠道微生物群、BSH 活性和胆汁酸代谢相关途径。体外，GOS 和 FUC 补充增加了干酪乳杆菌 DM8121 的 BSH 活性。
