Department of Clinical Laboratory, First Affiliated Hospital, Dalian Medical University, Dalian, China.
Department of Microecology, College of Basic Medical Sciences, Dalian Medical University, Dalian, China.
Front Cell Infect Microbiol. 2024 May 15;14:1333145. doi: 10.3389/fcimb.2024.1333145. eCollection 2024.
To investigate the structure, composition, and functions of the gut microbiota in elderly patients with hyperlipidemia.
Sixteen older patients diagnosed with hyperlipidemia (M group) and 10 healthy, age-matched normal volunteers (N group) were included. These groups were further subdivided by sex into the male normal (NM, n = 5), female normal (NF, n = 5), male hyperlipidemia (MM, n = 8), and female hyperlipidemia (MF, n = 8) subgroups. Stool samples were collected for high-throughput sequencing of 16S rRNA genes. Blood samples were collected for clinical biochemical index testing.
Alpha- and beta-diversity analyses revealed that the structure and composition of the gut microbiota were significantly different between the M and N groups. The relative abundances of , , , , and were significantly decreased, while those of , , and were significantly higher in the M group. There were also significant sex-related differences in microbial structure between the NM and NF groups, and between the MM and MF groups. Through functional prediction with PICRUSt 2, we observed distinct between-group variations in metabolic pathways associated with the gut microbiota and their impact on the functionality of the nervous system. Pearson's correlation coefficient was used as a distance metric to build co-abundance networks. A hypergeometric test was used to detect taxonomies with significant enrichment in specific clusters. We speculated that modules with and as the core microbes play an important ecological role in the intestinal microbiota of the M group. The relative intestinal abundances of and in the M group were positively correlated with serum triglyceride and low-density lipoprotein levels, while the relative abundance of was negatively correlated with the serum lipoprotein a level.
研究老年高血脂患者肠道微生物群的结构、组成和功能。
纳入 16 名老年高血脂患者（M 组）和 10 名健康、年龄匹配的正常志愿者（N 组）。这些组进一步按性别分为男性正常组（NM，n=5）、女性正常组（NF，n=5）、男性高血脂组（MM，n=8）和女性高血脂组（MF，n=8）亚组。采集粪便样本进行 16S rRNA 基因高通量测序。采集血液样本进行临床生化指标检测。
α-和β多样性分析表明，M 组和 N 组肠道微生物群的结构和组成存在显著差异。与 N 组相比，M 组中 、 、 、 和 的相对丰度显著降低，而 、 、 和 的相对丰度显著升高。NM 组和 NF 组以及 MM 组和 MF 组之间的微生物结构也存在显著的性别差异。通过 PICRUSt2 进行功能预测，我们观察到与肠道微生物群相关的代谢途径及其对神经系统功能的影响存在明显的组间差异。使用 Pearson 相关系数作为距离度量标准构建共丰度网络。使用超几何检验检测特定簇中具有显著富集的分类群。我们推测以 和 为核心微生物的模块在 M 组的肠道微生物群中发挥重要的生态作用。M 组中 和 的相对肠道丰度与血清甘油三酯和低密度脂蛋白水平呈正相关，而 的相对丰度与血清脂蛋白 a 水平呈负相关。
