Identification of two glycoproteins of chromaffin granules as the carboxypeptidase H.

An antiserum against carboxypeptidase H, an enzyme involved in the biosynthesis of neuropeptides such as the enkephalins, was used to identify the enzyme proteins in bovine chromaffin granules. Two-dimensional immuno- and lectin-blots revealed that two closely migrating glycoproteins which have been previously named J and K represented the enzyme. The same protein doublet was present in the membrane preparation and the soluble lysate of bovine chromaffin granules; however, the membrane contained significantly more enzyme protein.