McHugh S M, Coulson P S, Wilson R A
Parasitology. 1987 Feb;94 ( Pt 1):69-80. doi: 10.1017/s0031182000053464.
Alterations in the hepatic portal vasculature of NMRI mice infected with Schistosoma mansoni were assessed using a microsphere injection technique. The accumulation of eggs in the livers of infected mice and the development of portal hypertension were closely related to the worm pair burden during the first 15 weeks of infection. Individual variation between mice harbouring identical patent worm pair burdens was partially explained by the reduced fecundity of females from sexually biased infections. As eggs accumulated in the liver and portal hypertension increased, the number of injected microspheres escaping from the hepatic portal system rose in rank order of diameter from 9 microns through 15 microns and 25 microns to 50 microns. There was a strong correlation between the numbers of parasite eggs in the lungs and injected microspheres recovered from the lungs. The pattern of detection of microspheres in the lungs indicated a progressive increase in diameter of intra-hepatic porta-systemic connexions, followed by development of large-bore extra-hepatic collateral vessels. An accurate temporal profile of the pathological state of the host and the extent of collateral vessel formation was obtained. Injection of 141-Ce-labelled microspheres demonstrated that the arterial supply to all organs of the body was affected by alterations in the microvasculature of the liver and lungs.
采用微球注射技术评估感染曼氏血吸虫的NMRI小鼠肝门静脉系统的变化。感染小鼠肝脏中虫卵的聚集以及门静脉高压的发展与感染后前15周的虫荷密切相关。携带相同成虫虫荷的小鼠之间的个体差异部分可归因于性别偏向性感染导致的雌虫繁殖力下降。随着肝脏中虫卵的积累和门静脉高压的加剧，从肝门静脉系统逸出的注射微球数量按直径从小到大依次增加，从9微米到15微米、25微米再到50微米。肺中寄生虫卵数量与从肺中回收的注射微球数量之间存在很强的相关性。肺中微球的检测模式表明肝内门体连接直径逐渐增大，随后出现大口径肝外侧支血管。获得了宿主病理状态和侧支血管形成程度的准确时间概况。注射141-铈标记的微球表明，肝脏和肺微血管的改变会影响身体所有器官的动脉供血。
