Evans R, Booth C G, Spencer F
Br J Cancer. 1978 Nov;38(5):583-90. doi: 10.1038/bjc.1978.253.
Two transplantable, highly immunogenic syngeneic C57BL fibrosarcomas, FS1 and FS6, were shown to have tumour-specific rejection antigens, as shown by excision of the primary tumours and i.p. or i.m. injection of graded doses of the specific and unrelated tumour cells. I.p. challenge with tumour cells induced a large and relatively long-lasting increase in numbers of peritoneal leucocytes. Macrophage monolayers prepared from such exudates were, in general, non-specifically cytotoxic, though occasional specific cytotoxicity was detected. T lymphocytes isolated from exudates were shown to kill in a specific manner. When immunized mice were challenged with the specific tumour cells to elicit large numbers of peritoneal cytotoxic cells, and with graded doses of the non-cross-reacting tumour cells at the same time or at various times thereafter, growth of the non-related tumours occurred in all cases and only the specific tumour was rejected. Moreover, Winn tests, in which the inflammatory cells were mixed with unrelated tumour cells and implanted i.m., did not delay tumour growth. The relevance of these findings to the role of macrophages and lymphocytes in syngeneic tumour rejection is discussed.
两种可移植的、高度免疫原性的同基因C57BL纤维肉瘤FS1和FS6，已被证明具有肿瘤特异性排斥抗原，这通过切除原发性肿瘤以及腹腔内或肌肉内注射不同剂量的特异性和无关肿瘤细胞得以证实。用肿瘤细胞进行腹腔内攻击可导致腹腔白细胞数量大幅且相对持久地增加。通常，从这种渗出物制备的巨噬细胞单层具有非特异性细胞毒性，不过偶尔也能检测到特异性细胞毒性。从渗出物中分离出的T淋巴细胞显示出以特异性方式杀伤的能力。当用特异性肿瘤细胞攻击免疫小鼠以引发大量腹腔细胞毒性细胞时，同时或之后不同时间用不同剂量的非交叉反应肿瘤细胞攻击，所有情况下非相关肿瘤均生长，只有特异性肿瘤被排斥。此外，在Winn试验中，将炎性细胞与无关肿瘤细胞混合并肌肉内植入，并未延迟肿瘤生长。本文讨论了这些发现与巨噬细胞和淋巴细胞在同基因肿瘤排斥中的作用的相关性。
