Fritze D, Kern D H, Waldman S R, Pilch Y H
Int J Cancer. 1975 Jan 15;15(1):116-31. doi: 10.1002/ijc.2910150114.
Serological evidence is presented that two chemically-induced (by methylcholanthrene and 3,4-benz(a)pyrene) sarcomas of C3HF mice contain cross-reacting tumor-associated cell-surface antigens. Xenogeneic and syngeneic antitumor antisera against the two sarcomas were studied with an isotopic, complement-dependent, antibody-mediated microcytotoxicity assay and an isotopic antiglobulin test for the detection of antibodies to tumor-associated antigens, in vitro. Absorportions with various tissues were performed which consistently revealed that the specific activity of the antitumor antisera could be removed by absorption with cells from either chemically-induced tumor, while absorption with syngeneic normal adult tissues, normal fetal tissues, or cells from a histogenetically unrelated tumor (spontaneous mammary carcinoma) failed to remove any specific activity. In view of the individual character of carcinogen-induced tumor antigens as detected by tumor transplantation techniques, our results suggest that chemically-induced murine sarcomas (even when induced by different carcinogens) contain both private and common cell-surface antigens, the latter detectable by serological methods. These common tumor-associated antigens may be related to a viral genome involved in the nalignant transformation of carcinogen-induced marine sarcomas.
有血清学证据表明，C3HF小鼠的两种化学诱导（由甲基胆蒽和3,4-苯并(a)芘诱导）肉瘤含有交叉反应性肿瘤相关细胞表面抗原。利用同位素依赖补体的抗体介导的微细胞毒性试验和同位素抗球蛋白试验，在体外研究了针对这两种肉瘤的异种和同基因抗肿瘤抗血清，以检测针对肿瘤相关抗原的抗体。用各种组织进行吸收试验，结果始终表明，抗肿瘤抗血清的比活性可通过用来自任何一种化学诱导肿瘤的细胞进行吸收而去除，而用同基因正常成年组织、正常胎儿组织或来自组织发生学上无关肿瘤（自发性乳腺癌）的细胞进行吸收则不能去除任何比活性。鉴于通过肿瘤移植技术检测到的致癌物诱导肿瘤抗原的个体特征，我们的结果表明，化学诱导的小鼠肉瘤（即使由不同致癌物诱导）含有特异性和共同的细胞表面抗原，后者可用血清学方法检测到。这些共同的肿瘤相关抗原可能与参与致癌物诱导的小鼠肉瘤恶性转化的病毒基因组有关。
