KU Leuven Department of Microbiology and Immunology, Rega Institute for Medical Research, Laboratory of Virology and Chemotherapy, 3000 Leuven, Belgium.
Laboratory of Pathology, 3000 Leuven, Belgium.
Int J Mol Sci. 2019 Apr 29;20(9):2122. doi: 10.3390/ijms20092122.
In humans, Zika virus and viral RNA have been detected in semen up to 2.2 months and 6 months post infection (pi), respectively. Although the contribution of sexual transmission to the spread of ZIKV is too low to sustain an outbreak, it can increase the risk of infection and the epidemic size as well as prolong the duration of an outbreak. In this study, we explored the potential of antivirals to serve as an effective strategy to prevent sexual transmission. Male AG129 mice infected with a ZIKV isolate from Suriname were treated with the nucleoside analog, 7-deaza-2'--methyladenosine (7DMA), that was previously shown to be efficacious in reducing ZIKV viremia and delaying ZIKV-induced disease in mice. Following treatment, viral RNA and infectious virus titers were consistently reduced in the male reproductive organs compared to vehicle-treated mice. This reduction of ZIKV loads in the testis was confirmed by the detection of lower levels of ZIKV antigens. Our data illustrate the value of this mouse model to validate the efficacy of new potential ZIKV drugs at the level of the male reproductive system.
在人类中，寨卡病毒和病毒 RNA 分别在感染后 2.2 个月和 6 个月的精液中被检测到。尽管性传播对寨卡病毒的传播贡献太低，不足以维持疫情爆发，但它可以增加感染风险和疫情规模，并延长疫情持续时间。在这项研究中，我们探讨了抗病毒药物作为预防性传播的有效策略的潜力。感染苏里南分离株寨卡病毒的雄性 AG129 小鼠用先前显示在减少寨卡病毒血症和延迟小鼠寨卡病毒诱导疾病方面有效的核苷类似物 7-脱氮-2'-C-甲基腺苷（7DMA）进行治疗。与用载体治疗的小鼠相比，治疗后雄性生殖器官中的病毒 RNA 和传染性病毒滴度持续降低。通过检测寨卡病毒抗原水平较低，证实了睾丸中寨卡病毒载量的降低。我们的数据说明了这种小鼠模型在雄性生殖系统水平上验证新的潜在寨卡病毒药物疗效的价值。
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