Department of Gynecology Oncology, Xuzhou Cancer Hospital, Xuzhou Hospital Affiliated to Jiangsu University, Xuzhou, 221000 Jiangsu, China.
Cell Biol Int. 2019 Jul;43(7):781-788. doi: 10.1002/cbin.11152. Epub 2019 May 20.
Cervical cancer (CC) is the fourth leading cause of cancer-related death in women worldwide. There is an urgent need to find novel targets for the treatment of CC. Recently, microRNA have emerged as critical factors in tumorigenesis. In this study, we aimed to investigate the mechanism of miR-641 on the migration and invasion of CC cells. In silico analysis revealed putative interaction between miR-641 and phosphatase and tensin homolog (PTEN) RNA/lncRNA tumor suppressor candidate 8 (TUSC8). Hence we evaluated the expression of TUSC8, miR-641, and PTEN. We found that the expressions of TUSC8 and PTEN were decreased in CC tissues, whereas miR-641 expression was increased. Inhibition of miR-641 suppressed the migration and invasion of Hela cells. In addition, TUSC8 and PTEN were upstream and downstream target molecule of miR-641, respectively. Overexpression of TUSC8 promoted PTEN expression, and suppressed the invasion and migration of Hela cells, whereas miR-641 mimic treatment changed the effects. These results demonstrated that overexpression of TUSC8 could inhibit the invasion and migration of CC cells by upregulating PTEN via miR-641.
宫颈癌（CC）是全球女性癌症相关死亡的第四大原因。迫切需要寻找治疗 CC 的新靶点。最近，miRNA 已成为肿瘤发生的关键因素。在这项研究中，我们旨在研究 miR-641 对 CC 细胞迁移和侵袭的作用机制。计算机分析显示 miR-641 与磷酸酶和张力蛋白同源物（PTEN）RNA/lncRNA 肿瘤抑制候选物 8（TUSC8）之间存在潜在的相互作用。因此，我们评估了 TUSC8、miR-641 和 PTEN 的表达。我们发现 TUSC8 和 PTEN 在 CC 组织中表达降低，而 miR-641 表达增加。抑制 miR-641 可抑制 Hela 细胞的迁移和侵袭。此外，TUSC8 和 PTEN 分别是 miR-641 的上游和下游靶分子。过表达 TUSC8 可通过 miR-641 促进 PTEN 表达，抑制 Hela 细胞的侵袭和迁移，而 miR-641 模拟物处理则改变了这种作用。这些结果表明，TUSC8 的过表达可以通过 miR-641 上调 PTEN 来抑制 CC 细胞的侵袭和迁移。
