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伴刀豆球蛋白A在积液中癌细胞、组织细胞和间皮细胞上的分布模式

Distribution pattern of concanavalin A on carcinoma cells, histiocytes and mesothelial cells from effusions.

作者信息

Lubínski J

出版信息

Acta Cytol. 1987 Mar-Apr;31(2):99-103.

PMID:3103357
Abstract

Fixed and unfixed cancer cells, mesothelial cells and histiocytes were exposed to fluorescein-labeled concanavalin A (ConA-FITC). Cancer cells, whether fixed or unfixed, showed a similar pattern of fluorescence, as a continuous layer over the whole periphery of the cell. This pattern of ConA-FITC distribution was also obtained on fixed mesothelial cells and fixed histiocytes. Redistribution of ConA-FITC in form of "caps" and "patches" was recorded on unfixed mesothelial cells and unfixed histiocytes. Of the three cell types studied, only the histiocytes were lysed by the incubation with ConA-FITC.

摘要

将固定和未固定的癌细胞、间皮细胞和组织细胞暴露于荧光素标记的伴刀豆球蛋白A(ConA-FITC)中。癌细胞,无论是否固定,都呈现出相似的荧光模式,即细胞整个周边上的连续层。在固定的间皮细胞和固定的组织细胞上也获得了这种ConA-FITC分布模式。在未固定的间皮细胞和未固定的组织细胞上记录到ConA-FITC以“帽”和“斑块”形式的重新分布。在所研究的三种细胞类型中,只有组织细胞在与ConA-FITC孵育后被裂解。

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J Clin Pathol. 1989 Nov;42(11):1178-83. doi: 10.1136/jcp.42.11.1178.