Pavoni Gomes Chagas Thiago, da Silva Caldeira Luís Felipe, Vidal Pedinotti Zuma Alexandra, Cassiano de Oliveira Ivson, D'Alincourt Carvalho-Assef Ana Paula, Dutra Asensi Marise, Piffano Costa Pellegrino Flávia Lúcia
Departamento de Patologia, Faculdade de Medicina, Universidade Federal Fluminense, Niterói, Rio de Janeiro, Brazil.
Laboratórios Integrados de Pesquisa em Bactérias Resistentes aos Antimicrobianos, Unidade de Farmácia, Centro Universitário Estadual da Zona Oeste-UEZO, Campo Grande, Rio de Janeiro, Brazil.
New Microbiol. 2019 Apr;42(2):121-124. Epub 2019 Apr 29.
Because of the high prevalence of CTX-M-15-producing Escherichia coli isolates causing urinary tract infections in Rio de Janeiro, we have investigated bla-CTX-M-15 gene presence, as well as CTX-M-15 production, in 32 E. coli isolates recovered from the urine of outpatients assisted at a public hospital located in the west zone of Rio. Molecular epidemiology was assessed by PFGE and phylo-typing methods. The work highlights the good performance of MALDI-TOF MS as an alternative tool to detect extended-spectrum beta-lactamases among CTX-M-15-producing E. coli isolates.
由于在里约热内卢导致尿路感染的产CTX-M-15大肠杆菌分离株的高流行率，我们调查了从里约西区一家公立医院门诊患者尿液中分离出的32株大肠杆菌中bla-CTX-M-15基因的存在情况以及CTX-M-15的产生情况。通过脉冲场凝胶电泳（PFGE）和系统发育分型方法评估分子流行病学。这项工作突出了基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）作为检测产CTX-M-15大肠杆菌分离株中广谱β-内酰胺酶的替代工具的良好性能。
