Department of Veterinary Internal Medicine, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo-ku, Tokyo, Japan.
Anicom Specialty Medical Institute Inc., Shinjuku-ku, Tokyo, Japan.
PLoS One. 2019 Apr 29;14(4):e0208567. doi: 10.1371/journal.pone.0208567. eCollection 2019.
Exosomes are small extracellular vesicles released from almost all cell types, which play roles in cell-cell communication. Recent studies have suggested that microenvironmental crosstalk mediated by exosomes is an important factor in the escape of tumour cells from the anti-tumour immune system in human haematopoietic malignancies. Here, we conducted comprehensive analysis of the miRNA and protein profiles within the exosomes released from four canine lymphoid tumour cell lines as a model of human lymphoid tumours. The results showed that the major miRNAs and proteins extracted from the exosomes were similar among the four cell lines. However, the miRNA profiles differed among the exosomes of each cell line, which corresponded to the expression patterns of the parent cells. In the comparison of the amounts of miRNAs and proteins among the cell lines, those of three miRNAs (miR-151, miR-8908a-3p, and miR-486) and CD82 protein differed between exosomes derived from vincristine-sensitive and resistant cell lines. Further investigations are needed to elucidate the biological functions of the exosomal contents in the microenvironmental crosstalk of lymphoid tumours.
外泌体是几乎所有细胞类型释放的小型细胞外囊泡，在细胞间通讯中发挥作用。最近的研究表明，外泌体介导的微环境串扰是人类造血恶性肿瘤中肿瘤细胞逃避抗肿瘤免疫系统的重要因素。在这里，我们对四种犬淋巴肿瘤细胞系释放的外泌体中的 miRNA 和蛋白质谱进行了全面分析，作为人类淋巴肿瘤的模型。结果表明，四种细胞系来源的外泌体中提取的主要 miRNA 和蛋白质相似。然而，每个细胞系的外泌体中的 miRNA 图谱不同，与亲代细胞的表达模式相对应。在细胞系之间的 miRNA 和蛋白质含量的比较中，三种 miRNA（miR-151、miR-8908a-3p 和 miR-486）和 CD82 蛋白在长春新碱敏感和耐药细胞系来源的外泌体之间存在差异。需要进一步研究来阐明淋巴肿瘤微环境串扰中外泌体内容物的生物学功能。
