Haynes J D, Vernes A, Lyon J A
Am J Trop Med Hyg. 1987 Mar;36(2):246-56. doi: 10.4269/ajtmh.1987.36.246.
Immune sera from some Cambodian refugees contain functional serotypic antibodies that inhibit invasion of erythrocytes by the Camp strain but not by the FCR-3 strain of Plasmodium falciparum. Using a new assay, the "competitive heterologous antigen assay" (CHAA), the serotypic antibodies in a pool of three inhibitory sera were characterized by the antigens they precipitated. In the CHAA, immunoprecipitation of antigens by antibodies to common or cross-reacting antigenic determinants was blocked with excess heterologous unlabeled FCR-3 antigens before 3H-labeled Camp schizont and merozoite antigens were immunoprecipitated. The predominant Camp strain serotypic antigens revealed after electrophoresis and autoradiography were the major 195 Kd glycoprotein surface antigen (gp195) and its processed products at 150, 83, 73, and possibly 45 Kd. Additional serotypic antigens were identified at 180, 130, 65, 50, and 32 Kd. It is likely that one or more of these serotypic antigens is a target for the serotypic antibodies that inhibit invasion.
一些柬埔寨难民的免疫血清中含有功能性血清型抗体，这些抗体可抑制坎普株疟原虫对红细胞的入侵，但不能抑制恶性疟原虫FCR - 3株对红细胞的入侵。使用一种新的检测方法，即“竞争性异源抗原检测法”（CHAA），通过三种抑制性血清混合物中的血清型抗体沉淀的抗原对其进行了鉴定。在CHAA中，在用3H标记的坎普株裂殖体和裂殖子抗原进行免疫沉淀之前，先用过量的异源未标记FCR - 3抗原阻断针对共同或交叉反应抗原决定簇的抗体对抗原的免疫沉淀。电泳和放射自显影后显示的主要坎普株血清型抗原是主要的195 Kd糖蛋白表面抗原（gp195）及其150、83、73以及可能45 Kd的加工产物。在180、130、65、50和32 Kd处鉴定出了其他血清型抗原。这些血清型抗原中的一种或多种很可能是抑制入侵的血清型抗体的靶标。
