基于肽：MHCII四聚体的细胞富集用于表位特异性CD4 T细胞研究
Peptide:MHCII Tetramer-Based Cell Enrichment for the Study of Epitope-Specific CD4 T Cells.
作者信息
Kotov Dmitri I, Jenkins Marc K
机构信息
Department of Microbiology and Immunology, University of Minnesota, Minneapolis, Minnesota.
Center for Immunology, University of Minnesota, Minneapolis, Minnesota.
出版信息
Curr Protoc Immunol. 2019 Jun;125(1):e75. doi: 10.1002/cpim.75. Epub 2019 Apr 29.
Abstract
Epitope-specific CD4 T cells can be labeled in complex cell mixtures from secondary lymphoid organs with fluorophore-labeled peptide:major histocompatibility complex class II (p:MHCII) tetramers and then detected by flow cytometry. Magnetic enrichment of tetramer-bound cells before flow cytometry increases the sensitivity of detection to the point where epitope-specific cells can be studied even when very rare at early and late times after the host has been exposed to the epitope. This method is very useful for studying polyclonal epitope-specific CD4 T cells under physiological conditions. © 2019 by John Wiley & Sons, Inc.
摘要
表位特异性CD4 T细胞可以用荧光团标记的肽：主要组织相容性复合体II类（p:MHCII）四聚体在来自二级淋巴器官的复杂细胞混合物中进行标记，然后通过流式细胞术进行检测。在流式细胞术之前对与四聚体结合的细胞进行磁性富集，可将检测灵敏度提高到即使在宿主接触表位后的早期和晚期非常罕见时，也能研究表位特异性细胞的程度。该方法对于在生理条件下研究多克隆表位特异性CD4 T细胞非常有用。© 2019 John Wiley & Sons, Inc.
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