Park T H, Tachiki K H, Summers W K, Kling D, Fitten J, Perryman K, Spidell K, Kling A S
Anal Biochem. 1986 Dec;159(2):358-62. doi: 10.1016/0003-2697(86)90353-2.
Tacrine (THA; 1,2,3,4-tetrahydro-9-aminoacridine) is an anticholinesterase agent which has been used clinically, most recently in the treatment of Alzheimer-type dementias. This paper describes the methodology for the isolation and quantitation of THA at therapeutic levels in serum from human subjects. Using C18 Bond Elut columns and an HPLC/fluorometry system, this assay exhibits a considerable improvement in sensitivity over previous uv methods, and allows routine testing of THA levels in serum samples of reasonable volume from human subjects.
他克林（THA；1,2,3,4-四氢-9-氨基吖啶）是一种抗胆碱酯酶药物，已在临床上使用，最近用于治疗阿尔茨海默型痴呆症。本文描述了在人体受试者血清中分离和定量治疗水平的THA的方法。使用C18 Bond Elut柱和HPLC/荧光测定系统，该测定法与以前的紫外方法相比，灵敏度有了显著提高，并允许对来自人体受试者的适量血清样本中的THA水平进行常规检测。
