Pharmacokinetics and Drug Metabolism, Amgen Research, 1120 Veterans Blvd, S. San Francisco, CA 94080, USA.
Clinical Biomarkers and Diagnostics, Amgen Research, 1120 Veterans Blvd, S. San Francisco, CA 94080, USA.
J Immunol Methods. 2019 Jul;470:55-58. doi: 10.1016/j.jim.2019.04.010. Epub 2019 Apr 26.
FMS related tyrosine kinase 3 (FLT-3) is a tyrosine kinase expressed in early hematopoietic precursor cells and has roles in survival, proliferation, and differentiation. Bone marrow expression and mutagenic analysis of FLT-3 in Acute Myeloid Leukemia (AML) patients is well-characterized. However, the levels of circulating FLT-3 in serum have not been previously described. In this study we describe a quantitative electrochemiluminescent immunoassay that detects FLT-3 in human serum. Using this method we find that AML patients have elevated levels of circulating FLT-3 and these levels correlated to the percent blast counts in the bone marrow (BM).
FMS 相关酪氨酸激酶 3（FLT-3）是一种在早期造血前体细胞中表达的酪氨酸激酶，在生存、增殖和分化中发挥作用。骨髓中 FLT-3 的表达和突变分析在急性髓系白血病（AML）患者中已经得到很好的描述。然而，循环中的 FLT-3 水平以前没有描述过。在这项研究中，我们描述了一种定量电化学发光免疫分析方法，用于检测人血清中的 FLT-3。使用这种方法，我们发现 AML 患者的循环 FLT-3 水平升高，并且这些水平与骨髓中 blast 细胞的百分比相关。
