Russian Institute of Radiology and Agroecology, 249032, Obninsk, Russia; Institut Jean-Pierre Bourgin, INRA, AgroParisTech, CNRS, Université Paris-Saclay, 78000, Versailles, France.
Russian Institute of Radiology and Agroecology, 249032, Obninsk, Russia.
Environ Pollut. 2019 Jul;250:618-626. doi: 10.1016/j.envpol.2019.04.064. Epub 2019 Apr 21.
Radioactive contamination of the natural areas is one of the most long-lasting anthropogenic impacts on the environment. Scots pine (Pinus sylvestris L.) is a promising organism for radiation-related research because of its high radiosensitivity, but the genome size of Pinacea species has imposed obstacles for high-throughput studies so far. In this work, we conducted the analysis of the de novo assembled transcriptome of Scots pine populations growing in the Chernobyl-affected zone, which is still today contaminated with radionuclides because of the accident at the nuclear power plant in 1986. The transcriptome profiles indicate a clear pattern of adaptive stress response, which seems to be dose-dependent. The transcriptional response indicates a continuous modulation of the cellular redox system, enhanced expression of chaperones and histones, along with the control of ions balance. Interestingly, the activity of transposable element families is inversely correlated to the exposure levels to radiation. These adaptive responses, which are triggered by radiation doses 30 times lower than the one accepted as a safe for biota species by international regulations, suggest that the environmental management in radiation protection should be reviewed.
自然区域的放射性污染是对环境造成的最持久的人为影响之一。由于其高度的放射敏感性，欧洲赤松（Pinus sylvestris L.）是与辐射相关研究的有前途的生物体，但迄今为止，松科物种的基因组大小为高通量研究设置了障碍。在这项工作中，我们对生长在切尔诺贝利影响区的欧洲赤松种群的从头组装转录组进行了分析，由于 1986 年核电站事故，该地区至今仍受到放射性核素的污染。转录组谱表明存在明显的适应应激反应模式，似乎与剂量有关。转录反应表明细胞氧化还原系统的连续调节，伴侣蛋白和组蛋白的表达增强，以及离子平衡的控制。有趣的是，转座元件家族的活性与辐射暴露水平呈负相关。这些适应性反应是由辐射剂量引发的，其辐射剂量比国际法规所接受的生物物种安全剂量低 30 倍，这表明应重新审查辐射防护中的环境管理。
