[Correlation of Gene Mutations with the Protein Expressions of TAK1 and MAP4K2 in Colorectal Cancer].

OBJECTIVE

To analyze the correlation of gene mutations with the protein expressions of transforming growth factor-β activating kinase 1 (TAK1) protein and mitogen-activated protein kinase kinase kinase kinase 2 (MAP4K2) protein in colorectal cancer.

METHODS

gene mutations were detected by DNA sequencing analysis, and the expressions of TAK1 protein and MAP4K2 protein were detected by immunohistochemical method in 76 cases of colorectal cancer tissues.

RESULTS

In 76 cases of colorectal cancer tissues, the mutation rate of gene was 32.89% (25 cases), and gene mutations were correlated with the degrees of cell differentiation ( <0.05). The positive rates of TAK1 protein and MAP4K2 protein were 48.68% and 46.05%, respectively. The protein expressions of TAK1 and MAP4K2 were positively correlated with the degrees of cell differentiation and lymph node metastases, respectively ( <0.05). There was no correlation between gene mutation and either TAK1 protein or MAP4K2 protein expression ( >0.05). In 25 cases of colorectal cancer with mutation, the expression of TAK1 protein was positively correlated with the expression of MAP4K2 protein ( <0.05).

CONCLUSION

gene mutation, TAK1 and MAP4K2 protein expressions were related to the degree of differentiation of colorectal cancer, but not to the depth of invasion. In colorectal cancer with gene mutation, the expression of TAK1 protein was positively correlated with the expression of MAP4K2 protein.