microRNA-199a-5p 通过抑制 MAGT1 抑制神经胶质瘤的进展。
microRNA-199a-5p suppresses glioma progression by inhibiting MAGT1.
机构信息
Department of Neurosurgery, Chongqing Traditional Chinese Medicine Hospital, Chongqing, P.R. China.
Department of Neurosurgery, First Affiliated Hospital of Chongqing Medical University, Chongqing, P.R. China.
出版信息
J Cell Biochem. 2019 Sep;120(9):15248-15254. doi: 10.1002/jcb.28791. Epub 2019 Apr 30.
microRNAs (miRNAs) can function as a tumor suppressor or oncogenic genes in human cancers. Alternation expression of miR-199a-5p has been revealed in several human cancers. However, its expression pattern and biological roles in glioma remain unclear. Expression levels of miR-199a-5p in glioma were evaluated at first. The effects of miR-199a-5p expression on cell proliferation, migration, and invasion were investigated using the MTT assay, wound-healing assay, and transwell invasion assay. The expression of miR-199a-5p was found to be reduced in glioma cell lines. Overexpression of miR-199a-5p inhibits glioma cell proliferation, migration, and invasion in vitro. Furthermore, the target of miR-199a-5p was predicted by TargetScan and validated by luciferase activity reporter assay. We found magnesium transporter 1 (MAGT1) was a direct target of miR-199a-5p. Overexpression of MAGT1 reversed the effects of miR-199a-5p on glioma cell behaviors. Taken together, our study revealed that miR-199a-5p and MAGT1 have the potential to be used as a biomarker for glioma.
微小 RNA（miRNAs）可作为人类癌症中的肿瘤抑制基因或癌基因发挥作用。miR-199a-5p 的异常表达已在多种人类癌症中被揭示。然而，其在神经胶质瘤中的表达模式和生物学功能仍不清楚。本研究首先评估了 miR-199a-5p 在神经胶质瘤中的表达水平。通过 MTT 检测、划痕愈合检测和 Transwell 侵袭检测，研究了 miR-199a-5p 表达对细胞增殖、迁移和侵袭的影响。结果发现，miR-199a-5p 在神经胶质瘤细胞系中表达降低。miR-199a-5p 的过表达可抑制神经胶质瘤细胞在体外的增殖、迁移和侵袭。此外，通过 TargetScan 预测和荧光素酶活性报告基因检测验证了 miR-199a-5p 的靶基因。我们发现镁转运蛋白 1（MAGT1）是 miR-199a-5p 的直接靶基因。过表达 MAGT1 逆转了 miR-199a-5p 对神经胶质瘤细胞行为的影响。综上所述，我们的研究表明 miR-199a-5p 和 MAGT1 有可能成为神经胶质瘤的生物标志物。
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