微小 RNA-199a-5p 通过靶向 MARCH8 调节神经胶质瘤进展。
MicroRNA-199a-5p regulates glioma progression via targeting MARCH8.
机构信息
Department of Neurosurgery Ward 1, Hanzhong Central Hospital, Hanzhong, P.R. China.
出版信息
Eur Rev Med Pharmacol Sci. 2019 Sep;23(17):7482-7487. doi: 10.26355/eurrev_201909_18858.
OBJECTIVE
MicroRNA-199a-5p (miR-199a-5p) was reported to play crucial roles in cancer progression. However, its role in glioma remains largely unknown.
MATERIALS AND METHODS
RT-qPCR was employed to analyze miR-199a-5p expression level in glioma cell lines. The effects of miR-199a-5p on cell proliferation and invasion were investigated in vitro.
RESULTS
We showed that miR-199a-5p expression level was significantly downregulated in glioma cell lines compared with a normal cell. In addition, miR-199a-5p overexpression suppresses glioma cell proliferation and invasion in vitro. Bioinformatic analysis and Luciferase assay suggested that the membrane-associated ring-CH-type finger 8 (MARCH8) was a target of miR-199a-5p. Furthermore, MARCH8 overexpression could partially reverse the effects of miR-199a-5p on glioma cells.
CONCLUSIONS
These findings suggested that miR-199a-5p may function as a tumor suppressor in glioma by targeting MARCH8.
目的
MicroRNA-199a-5p（miR-199a-5p）在癌症进展中发挥着至关重要的作用。然而，其在神经胶质瘤中的作用仍知之甚少。
材料与方法
采用 RT-qPCR 分析神经胶质瘤细胞系中 miR-199a-5p 的表达水平。在体外研究 miR-199a-5p 对细胞增殖和侵袭的影响。
结果
我们发现与正常细胞相比，miR-199a-5p 在神经胶质瘤细胞系中的表达水平显著下调。此外，miR-199a-5p 的过表达可抑制体外神经胶质瘤细胞的增殖和侵袭。生物信息学分析和荧光素酶报告基因实验表明，膜相关环-CH 型手指 8（MARCH8）是 miR-199a-5p 的靶基因。此外，MARCH8 的过表达可以部分逆转 miR-199a-5p 对神经胶质瘤细胞的作用。
结论
这些发现表明，miR-199a-5p 可能通过靶向 MARCH8 在神经胶质瘤中发挥肿瘤抑制作用。
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