Mustelin T
FEBS Lett. 1987 Mar 9;213(1):199-203. doi: 10.1016/0014-5793(87)81491-6.
The T3 molecule on the surface membrane of T lymphocytes is involved in the transduction of the proliferation signal generated by an interaction between the antigen receptor and an antigen, to the interior of the T cell. Mitogenic monoclonal antibodies against the T3 molecule and mitogenic lectins induce a rapid (within 5 min) protein synthesis-independent activation of ornithine decarboxylase (ODC) in human T lymphocytes. When T cells are selectively depleted of guanine nucleotides by treatment with mycophenolic acid, the early mitogen-induced activation of ODC is completely inhibited. The inhibition rapidly reverted on the addition of guanine a few minutes before the mitogenic stimulation, and even more rapidly by GTP directly introduced into the T cells by a transient membrane permeabilization. GTP can be substituted for by a non-hydrolyzable GTP analogue, GTP-gamma-S, which also induces ODC activity by itself in human T cells. These results suggest that a G-protein(s) is involved in the transduction of the proliferation signal in human T cells.
T淋巴细胞表面膜上的T3分子参与将抗原受体与抗原相互作用产生的增殖信号转导至T细胞内部。抗T3分子的促有丝分裂单克隆抗体和促有丝分裂凝集素可在人T淋巴细胞中诱导鸟氨酸脱羧酶（ODC）快速（5分钟内）的非蛋白质合成依赖性激活。当用霉酚酸处理使T细胞中的鸟嘌呤核苷酸选择性耗竭时，早期促有丝分裂原诱导的ODC激活被完全抑制。在促有丝分裂刺激前几分钟加入鸟嘌呤，抑制作用迅速逆转，通过短暂的膜通透将GTP直接导入T细胞，逆转速度更快。GTP可被不可水解的GTP类似物GTP-γ-S替代，GTP-γ-S自身也可在人T细胞中诱导ODC活性。这些结果表明，一种G蛋白参与了人T细胞增殖信号的转导。
