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苯并（a）芘二醇环氧化物-DNA加合物与CHO/HPGRT试验中致突变性之间的关系。

The relationship between benzo(a)pyrene diol-epoxide-DNA adducts and mutagenicity in the CHO/HPGRT assay.

作者信息

Recio L, Shugart L R, Hsie A W

出版信息

Fundam Appl Toxicol. 1987 Feb;8(2):243-52. doi: 10.1016/0272-0590(87)90123-0.

DOI:10.1016/0272-0590(87)90123-0
PMID:3104121
Abstract

We have studied the relationship between DNA adducts in Chinese hamster ovary (CHO) cells and mutagenicity as determined in the CHO/hypoxanthine-guanine phosphoribosyltransferase assay. The cells were treated with benzo(a)pyrene 7,8-diol (BP-diol) in the presence of a bioactivation system, S9 mix. DNA binding by bioactivation of BP-diol with S9 mix occurred with both stereoisomers of benzo(a)pyrene diol-epoxide (BPDE) in approximately equal amounts. The number of BPDE-DNA adducts (21-260 adducts/10(6) nucleotide base pairs) increased with increasing treatment concentrations of BP-diol (1.4-7.0 microM). A linear relationship was observed between the number of BPDE-DNA adducts and mutagenicity (89-605 mutants/10(6) cloneable cells) over the concentration range of BP-diol assayed.

摘要

我们研究了中国仓鼠卵巢（CHO）细胞中的DNA加合物与在CHO/次黄嘌呤-鸟嘌呤磷酸核糖转移酶试验中测定的致突变性之间的关系。细胞在生物活化系统S9混合物存在的情况下用苯并(a)芘7,8-二醇（BP-二醇）处理。BP-二醇与S9混合物进行生物活化导致的DNA结合在苯并(a)芘二醇环氧化物（BPDE）的两种立体异构体中以大致相等的量发生。BPDE-DNA加合物的数量（21 - 260个加合物/10⁶个核苷酸碱基对）随着BP-二醇处理浓度（1.4 - 7.0 microM）的增加而增加。在所测定的BP-二醇浓度范围内，观察到BPDE-DNA加合物的数量与致突变性（89 - 605个突变体/10⁶个可克隆细胞）之间存在线性关系。

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