枯草芽孢杆菌中无 CCA 酶时 tRNA 的加工分析。
Analysis of tRNA processing in the absence of CCAase in Bacillus subtilis.
机构信息
Facultad de Química, Universidad Autónoma de Querétaro, Cerro de las Campanas s/n, 76010, Querétaro, QRO, Mexico.
Secretaria de Investigación y Posgrado, Centro de Innovación y Transferencia de Tecnología (CENIT2), Universidad Autónoma de Nayarit, Tepic, Mexico.
出版信息
Braz J Microbiol. 2019 Jul;50(3):613-618. doi: 10.1007/s42770-019-00075-5. Epub 2019 Apr 12.
Abstract
In Bacillus subtilis, the tRNA lacks an encoded CCA 3' end. To gain insight into the role of CCAase and RNases in tRNA processing, several mutant strains were generated. Northern blot and RT-PCR results suggest that enzymes other than CCAase can participate in CCA addition at the 3' end of the immature tRNA.
摘要
在枯草芽孢杆菌中，tRNA 缺乏编码的 CCA 3'末端。为了深入了解 CCAase 和 RNases 在 tRNA 加工中的作用，生成了几种突变株。Northern blot 和 RT-PCR 结果表明，除了 CCAase 之外的酶也可以参与不成熟 tRNA 3'末端的 CCA 添加。
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本文引用的文献
1
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2
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Metallomics. 2013 Apr;5(4):398-403. doi: 10.1039/c3mt20203h.
3
Characterization of tRNA(Cys) processing in a conditional Bacillus subtilis CCase mutant reveals the participation of RNase R in its quality control.
Microbiology (Reading). 2010 Jul;156(Pt 7):2102-2111. doi: 10.1099/mic.0.034652-0. Epub 2010 Apr 1.
4
Addition of poly(A) and heteropolymeric 3' ends in Bacillus subtilis wild-type and polynucleotide phosphorylase-deficient strains.
J Bacteriol. 2005 Jul;187(14):4698-706. doi: 10.1128/JB.187.14.4698-4706.2005.
5
Ribonuclease PH plays a major role in the exonucleolytic maturation of CCA-containing tRNA precursors in Bacillus subtilis.
Nucleic Acids Res. 2005 Jun 27;33(11):3636-43. doi: 10.1093/nar/gki675. Print 2005.
6
Participation of 3'-to-5' exoribonucleases in the turnover of Bacillus subtilis mRNA.
J Bacteriol. 2005 Apr;187(8):2758-67. doi: 10.1128/JB.187.8.2758-2767.2005.
7
Endonucleolytic processing of CCA-less tRNA precursors by RNase Z in Bacillus subtilis.
EMBO J. 2003 Sep 1;22(17):4534-43. doi: 10.1093/emboj/cdg435.
8
Bacillus subtilis YhaM, a member of a new family of 3'-to-5' exonucleases in gram-positive bacteria.
J Bacteriol. 2002 Nov;184(22):6250-9. doi: 10.1128/JB.184.22.6250-6259.2002.
9
Polynucleotide phosphorylase functions both as a 3' right-arrow 5' exonuclease and a poly(A) polymerase in Escherichia coli.
Proc Natl Acad Sci U S A. 2000 Oct 24;97(22):11966-71. doi: 10.1073/pnas.220295997.
10
Functional overlap of tRNA nucleotidyltransferase, poly(A) polymerase I, and polynucleotide phosphorylase.
J Biol Chem. 1997 Dec 26;272(52):33255-9. doi: 10.1074/jbc.272.52.33255.
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