质谱分析证据表明精囊环氧化酶可将外源性肾上腺素转化为双高前列腺素。两种动物物种的比较研究。
Mass spectrometric evidence for the conversion of exogenous adrenate to dihomo-prostaglandins by seminal vesicle cyclo-oxygenase. A comparative study of two animal species.
作者信息
Ferretti A, Flanagan V P
出版信息
J Chromatogr. 1986 Dec 19;383(2):241-50. doi: 10.1016/s0378-4347(00)83470-2.
We present conclusive mass spectrometric evidence for the synthesis of 1a,1b-dihomoprostaglandin E2 and 1a,1b-dihomoprostaglandin F2 alpha by sheep seminal vesicle microsomes and swine whole vesicular homogenates. Swine microsomes prepared according to standard procedure were catalytically inactive, possibly due to autoinactivation of the cyclo-oxygenase caused by metabolism of endogenous substrate released during the isolation procedure. Cyclo-oxygenase activity could be demonstrated, however, when the porcine vesicles were homogenized in the presence of exogenous substrate. Chemical and mass spectrometric evidence presented here allow unequivocal structural assignments.
我们提供了确凿的质谱证据，证明绵羊精囊微粒体和猪全囊泡匀浆可合成1a,1b-二高前列腺素E2和1a,1b-二高前列腺素F2α。按照标准程序制备的猪微粒体没有催化活性，这可能是由于在分离过程中释放的内源性底物代谢导致环氧化酶自动失活。然而，当猪囊泡在外源底物存在下匀浆时，可证明环氧化酶活性。本文提供的化学和质谱证据允许进行明确的结构归属。
Zotero 插件