Vergara J, Bezanilla F, Salzberg B M
J Gen Physiol. 1978 Dec;72(6):775-800. doi: 10.1085/jgp.72.6.775.
A method is presented for recording extrinsic optical signals from segments of single skeletal muscle fibers under current or voltage clamp conditions. Such segments, which are cut from intact fibers, are maintained in a relaxed state, while exhbiting otherwise normal physiological properties, including healthy delayed rectifier currents. Extrinsic fluorescence changes are demonstrated, using the permeant potentiometric probe, Nile Blue A. These changes vary nonlinearly with the controlled surface membrane potential, in a manner which suggests that they arise from potential changes in the sarcoplasmic reticulum. According to this interpretation, a simple model based on the gating charge movement implicated in excitation-contraction coupling, provides a self-consistent description of the voltage dependence of the signal that requires no additional parameters.
本文介绍了一种在电流钳或电压钳条件下记录单个骨骼肌纤维片段外在光学信号的方法。这些从完整纤维上切下的片段保持松弛状态，同时表现出正常的生理特性，包括正常的延迟整流电流。使用渗透性电位探针尼罗蓝A证明了外在荧光变化。这些变化与受控的表面膜电位呈非线性变化，这表明它们源于肌浆网中的电位变化。根据这种解释，基于兴奋-收缩偶联中涉及的门控电荷运动的简单模型，对信号的电压依赖性提供了自洽的描述，无需额外参数。
