Analytical Biochemistry Research Centre (ABrC), Universiti Sains Malaysia, 11800 USM, Penang, Malaysia.
Department of Chemistry, Faculty of Applied Sciences, Universiti Teknologi MARA, Perlis Branch, 02600 Arau, Perlis, Malaysia.
Carbohydr Polym. 2019 Jul 15;216:303-311. doi: 10.1016/j.carbpol.2019.04.007. Epub 2019 Apr 6.
The Box-Behnken design was applied to optimize the extraction of pectin from Averrhoa bilimbi (ABP) using deep eutectic solvents (DES). The four variables of extraction were percentage of DES (X), extraction time (X), temperature (X), and molar ratio of DES components (X). The quadratic regression equation was established as a predicted model with R value of 0.9375. The optimal condition was X = 3.74% (w/v), X = 2.5 h, X = 80 °C, and X = 1:1. No significant difference between the predicted (14.70%) and experimental (14.44%) maximum yield of sample was noted. Characterization of physico-chemical properties characterization of ABP was performed. The main components of ABP were galacturonic acids, arabinoses, and xyloses. ABP also showed good functional properties such as water holding capacity (3.70 g/g), oil holding capacity (2.40 g/g), and foaming capacity (133.33%). The results also showed that ABP exhibited free radical scavenging activity (41.46%) and ferric reducing antioxidant power (1.15 mM).
Box-Behnken 设计被应用于优化使用深共晶溶剂 (DES) 从攀援酸模叶蓼 (ABP) 中提取果胶。提取的四个变量为 DES 的百分比 (X)、提取时间 (X)、温度 (X) 和 DES 成分的摩尔比 (X)。建立了二次回归方程作为预测模型，R 值为 0.9375。最佳条件为 X=3.74%（w/v）、X=2.5 h、X=80°C 和 X=1:1。未注意到预测（14.70%）和实验（14.44%）最大产率之间的显著差异。对 ABP 的物理化学性质进行了表征。ABP 的主要成分是半乳糖醛酸、阿拉伯糖和木糖。ABP 还表现出良好的功能性质，如持水力（3.70 g/g）、持油力（2.40 g/g）和起泡能力（133.33%）。结果还表明，ABP 表现出自由基清除活性（41.46%）和铁还原抗氧化能力（1.15 mM）。
