Gunter K C, Germain R N, Kroczek R A, Saito T, Yokoyama W M, Chan C, Weiss A, Shevach E M
Nature. 1987;326(6112):505-7. doi: 10.1038/326505a0.
In addition to monoclonal antibodies against the CD3 (T3)-T-cell antigen receptor (CD3/Ti) complex, several other monoclonals directed towards distinct cell surface structures on human (CD2 (T11) and Tp44) and murine (Thy-1, TAP, and Ly-6) T lymphocytes are capable of activating T cells. It has been proposed that such structures may function as alternative pathways of stimulation. To examine directly whether any relationship exists between Thy-1-dependent activation phenomena and T-cell activation mediated through the CD3/Ti complex, we have transfected several CD3/Ti- variants of the human T-cell line Jurkat with the murine Thy-1.2 gene. Our data indicate that in CD3/Ti-, Thy-1.2+ transfectants, monoclonal antibodies against Thy-1.2 can induce a rise in cytoplasmic free calcium ([Ca2+]i), but fail to stimulate interleukin-2 (IL-2) production. The only defect in these variant cell lines responsible for the inability to produce IL-2 in response to Thy-1 stimulation was in the expression of the CD3/Ti complex, because replacement of defective Ti alpha- or beta-chain genes reconstributed both surface expression of CD3/Ti and responsiveness to Thy-1 in the IL-2 production assay.
除了针对CD3（T3）-T细胞抗原受体（CD3/Ti）复合物的单克隆抗体外，其他几种针对人（CD2（T11）和Tp44）和鼠（Thy-1、TAP和Ly-6）T淋巴细胞上不同细胞表面结构的单克隆抗体也能够激活T细胞。有人提出，这些结构可能作为替代刺激途径发挥作用。为了直接研究Thy-1依赖性激活现象与通过CD3/Ti复合物介导的T细胞激活之间是否存在任何关系，我们用鼠Thy-1.2基因转染了人T细胞系Jurkat的几种CD3/Ti变体。我们的数据表明，在CD3/Ti-、Thy-1.2+转染子中，针对Thy-1.2的单克隆抗体可诱导细胞质游离钙（[Ca2+]i）升高，但不能刺激白细胞介素-2（IL-2）的产生。这些变体细胞系中唯一导致无法对Thy-1刺激产生IL-2的缺陷在于CD3/Ti复合物的表达，因为在IL-2产生试验中，替换有缺陷的Tiα或β链基因可恢复CD3/Ti的表面表达和对Thy-1的反应性。
