Effect of ginsenoside Rh on renal apoptosis in cisplatin-induced nephrotoxicity in vivo.

BACKGROUND

Ginsenoside Rh (Rh), an important ingredient from Panax ginseng, has received much attention due to a range of pharmacological actions.

PURPOSE

The aim of the study was to investigate the therapeutic potential Rh on cisplatin (CDDP)-induced nephrotoxicity and to elucidate involved mechanisms.

STUDY DESIGN

An in vivo mice model of CDDP-induced nephrotoxicity was established by a single intraperitoneal injection of CDDP (20 mg/kg) to assess the effects of Rh on renal biochemical parameter, oxidative stress, inflammation tubular cell apoptosis and serum metabolic profiles.

RESULTS

Rh protected against CDDP-induced renal dysfunction and ameliorated CDDP-induced oxidative stress, histopathological damage, inflammation and tubular cell apoptosis in kidney. Rh treatment had significantly increased expression of Bcl-2 and decreased expression of p53, Bax, cytochrome c, caspase-8, caspase-9, and caspase-3 in kidney tissues. Metabolomic analysis identified 29 altered serum metabolites in Rh treatment mice.

CONCLUSION

These results suggest that Rh protects against CDDP-induced nephrotoxicity via action on caspase-mediated pathway.