Wang Sheng, Wang Zigui, Wang Qiming, Cui Yun, Luo Suxia
The Affiliated Cancer Hospital of Zhengzhou University, Zhengzhou, Henan, China.
The Second People's Hospital of Nanzhao, Nanzhao, Henan, China.
Saudi J Biol Sci. 2019 May;26(4):777-781. doi: 10.1016/j.sjbs.2018.12.009. Epub 2018 Dec 17.
To explore the expression differences of miRNA-21, miRNA-31 and miRNA-let7 between lung cancer patient and healthy people, thereby providing reference for early diagnosis of lung cancer.
Real-time PCR was employed to determine the expression difference between lung cancer patients (50 cases) and healthy people (24 cases). The clinical data of lung cancer patients were analyzed to explore the correlation between clinicopathological characteristics and expression level of miRNA-21, miRNA-31, miRNA-let7.
The relative expression levels of miRNA-21 and miRNA-31 in lung cancer group were obviously higher than those in healthy control group, and the relative expression level of miRNA-let7 in lung cancer group was slightly higher than that in healthy control group. Lung cancer patients with lymph node metastasis had higher expression level than those without lymph node metastasis. The ROC curve showed that the three miRNAs had clinical diagnosis efficiency for lung cancer, and the combined detection of the three miRNAs were more efficient in diagnosing lung cancer. Survival curve analysis suggested that the median survival times of patients in the miRNA-21 and miRNA-31 high expression groups were shorter than those in the low expression groups, and the median survival time of patients in miRNA-let7 high expression group was longer than that in the low expression group.
Plasma miRNA-21, miRNA-31 and miRNA-let7 may be diagnostic marker for lung cancer.
探讨肺癌患者与健康人之间miRNA-21、miRNA-31和miRNA-let7的表达差异，从而为肺癌的早期诊断提供参考。
采用实时荧光定量聚合酶链反应（Real-time PCR）检测50例肺癌患者和24例健康人的表达差异。分析肺癌患者的临床资料，探讨miRNA-21、miRNA-31、miRNA-let7的表达水平与临床病理特征之间的相关性。
肺癌组miRNA-21和miRNA-31的相对表达水平明显高于健康对照组，肺癌组miRNA-let7的相对表达水平略高于健康对照组。有淋巴结转移的肺癌患者表达水平高于无淋巴结转移者。ROC曲线显示，这三种微小RNA对肺癌具有临床诊断效能，联合检测这三种微小RNA对肺癌的诊断效率更高。生存曲线分析表明，miRNA-21和miRNA-31高表达组患者的中位生存时间短于低表达组，miRNA-let7高表达组患者的中位生存时间长于低表达组。
血浆miRNA-21、miRNA-31和miRNA-let7可能是肺癌的诊断标志物。
