Yap Hooi-Yeen, Siow Thin-Sam, Chow Sook-Khuan, Teow Sin-Yeang
Department of Medical Sciences, School of Healthcare and Medical Sciences, Sunway University, Jalan Universiti, Bandar Sunway, 47500 Subang Jaya, Selangor Darul Ehsan, Malaysia.
Department of Biological Sciences, School of Science and Technology, Sunway University, Jalan Universiti, Bandar Sunway, 47500 Subang Jaya, Selangor Darul Ehsan, Malaysia.
Adv Virol. 2019 Mar 28;2019:6464521. doi: 10.1155/2019/6464521. eCollection 2019.
Epstein-Barr virus (EBV) is one of the common human herpesvirus types in the world. EBV is known to infect more than 95% of adults in the world. The virus mainly infects B lymphocytes and could immortalize and transform the cells into EBV-bearing lymphoblastoid cell lines (LCLs). Limited studies have been focused on characterizing the surface marker expression of the immortalized LCLs. This study demonstrates the generation of 15 LCLs from sixteen rheumatoid arthritis (RA) patients and a healthy volunteer using B95-8 marmoset-derived EBV. The success rate of LCL generation was 88.23%. All CD19+ LCLs expressed CD23 (16.94-58.9%) and CD27 (15.74-80.89%) on cell surface. Our data demonstrated two distinct categories of LCLs (fast- and slow-growing) (<0.05) based on their doubling time. The slow-growing LCLs showed lower CD23 level (35.28%) compared to fast-growing LCLs (42.39%). In contrast, the slow-growing LCLs showed higher percentage in both CD27 alone and CD23+CD27+ in combination. Overall, these findings may suggest the correlations of cellular CD23 and CD27 expression with the proliferation rate of the generated LCLs. Increase expression of CD23 may play a role in EBV immortalization of B-cells and the growth and maintenance of the EBV-transformed LCLs while CD27 expression might have inhibitory effects on LCL proliferation. Further investigations are warranted to these speculations.
爱泼斯坦-巴尔病毒（EBV）是世界上常见的人类疱疹病毒类型之一。已知EBV感染了世界上超过95%的成年人。该病毒主要感染B淋巴细胞，并可使细胞永生化并将其转化为携带EBV的淋巴母细胞系（LCLs）。有限的研究集中在对永生化LCLs的表面标志物表达进行表征。本研究展示了使用源自绒猴的B95-8 EBV从16名类风湿性关节炎（RA）患者和1名健康志愿者中生成15个LCLs。LCL生成的成功率为88.23%。所有CD19+ LCLs在细胞表面均表达CD23（16.94 - 58.9%）和CD27（15.74 - 80.89%）。我们的数据基于其倍增时间证明了两种不同类型的LCLs（生长快和生长慢）（<0.05）。与生长快的LCLs（42.39%）相比，生长慢的LCLs显示出较低的CD23水平（35.28%）。相反，生长慢的LCLs单独的CD27以及CD23+CD27+组合的百分比更高。总体而言，这些发现可能表明细胞CD23和CD27表达与所生成LCLs的增殖率之间的相关性。CD23表达的增加可能在B细胞的EBV永生化以及EBV转化的LCLs的生长和维持中起作用，而CD27表达可能对LCL增殖具有抑制作用。有必要对这些推测进行进一步研究。
