Leibniz-Institute for Food Systems Biology at the Technical University of Munich , Lise-Meitner-Straße 34 , 85354 Freising , Germany.
J Agric Food Chem. 2019 May 22;67(20):5838-5846. doi: 10.1021/acs.jafc.9b01445. Epub 2019 May 13.
An aroma extract dilution analysis applied to the volatiles isolated from jackfruit ( Artocarpus heterophyllus Lam.) pulp by solvent extraction and solvent-assisted flavor evaporation resulted in the detection of 48 odorants with flavor dilution (FD) factors between 1 and ≥8192. Application of gas chromatography-olfactometry to static headspace samples revealed additional five highly volatile odorants. The structures of 44 out of the 53 detected odorants could be assigned. These 44 compounds were quantitated using stable isotopically substituted odorants as internal standards, and their odor activity values (OAVs) were calculated as the ratio of the natural concentrations in jackfruit pulp and the odor threshold values in water. High OAVs were in particular obtained for ethyl 3-methylbutanoate (74000), ethyl butanoate (1800), 3-methylbutanal (1500), and 2-methylpropanal (1400). An aroma model solution based on the natural concentrations of the 35 compounds for which OAVs > 1 had been calculated fully mimicked the characteristic jackfruit pulp aroma.
采用香气提取稀释分析（AEDA）对溶剂萃取和溶剂辅助风味蒸发法从菠萝蜜（Artocarpus heterophyllus Lam.）果肉中分离出的挥发性成分进行分析，共检测到 48 种具有 1 至≥8192 稀释倍数（FD）的风味活性化合物。采用顶空固相微萃取-气相色谱-嗅闻技术（HS-SPME-GC-O）对静态顶空样品进行分析，发现了另外 5 种高挥发性气味物质。鉴定出 53 种气味物质中的 44 种，其中 44 种化合物采用稳定同位素内标法定量，其气味活性值（OAV）计算为菠萝蜜果肉中天然浓度与水中气味阈值的比值。具有高 OAV 的化合物包括：3-甲基丁酸乙酯（74000）、丁酸乙酯（1800）、3-甲基丁醛（1500）和 2-甲基丙醛（1400）。基于计算出的 OAV 值大于 1 的 35 种化合物的天然浓度，构建了一个香气模型溶液，能够完全模拟菠萝蜜果肉的典型香气。
