The Gurdon Institute, University of Cambridge, Cambridge, CB2 1QN, UK; Department of Genetics, University of Cambridge, Cambridge CB2 3EH, UK.
The Gurdon Institute, University of Cambridge, Cambridge, CB2 1QN, UK; Department of Genetics, University of Cambridge, Cambridge CB2 3EH, UK.
Mol Cell. 2019 May 2;74(3):598-608.e6. doi: 10.1016/j.molcel.2019.03.010. Epub 2019 Apr 18.
RNA flow between organisms has been documented within and among different kingdoms of life. Recently, we demonstrated horizontal RNA transfer between honeybees involving secretion and ingestion of worker and royal jellies. However, how the jelly facilitates transfer of RNA is still unknown. Here, we show that worker and royal jellies harbor robust RNA-binding activity. We report that a highly abundant jelly component, major royal jelly protein 3 (MRJP-3), acts as an extracellular non-sequence-specific RNA-aggregating factor. Multivalent RNA binding stimulates higher-order assembly of MRJP-3 into extracellular ribonucleoprotein granules that protect RNA from degradation and enhance RNA bioavailability. These findings reveal that honeybees have evolved a secreted dietary RNA-binding factor to concentrate, stabilize, and share RNA among individuals. Our work identifies high-order ribonucleoprotein assemblies with functions outside cells and organisms.
生物体之间的 RNA 流动已在不同的生命王国中得到证实。最近，我们证明了涉及工蜂和蜂王浆分泌和摄入的蜜蜂之间的水平 RNA 转移。然而， jelly 如何促进 RNA 的转移仍然未知。在这里，我们表明工蜂和蜂王浆具有强大的 RNA 结合活性。我们报告说，一种丰富的 jelly 成分，主要蜂王浆蛋白 3（MRJP-3），作为一种细胞外非序列特异性 RNA 聚集因子。多价 RNA 结合刺激 MRJP-3 形成更高阶的细胞外核糖核蛋白颗粒，从而保护 RNA 免受降解并提高 RNA 的生物利用度。这些发现表明，蜜蜂已经进化出一种分泌的饮食 RNA 结合因子，以在个体之间浓缩、稳定和共享 RNA。我们的工作确定了具有细胞和生物体外功能的高阶核糖核蛋白组装体。
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