UCN3与胆囊收缩素（CCK）及CCK2R协同作用，抑制西伯利亚鲟（Acipenser baerii）的食物摄取。
UCN3 suppresses food intake in coordination with CCK and the CCK2R in Siberian sturgeon (Acipenser baerii).
作者信息
Tang Ni, Zhang Xin, Wang Shuyao, Qi Jinwen, Tian Zhengzhi, Wang Bin, Chen Hu, Wu Yuanbing, Wang Mei, Xu Shaoqi, Chen Defang, Li Zhiqiong
机构信息
Department of Aquaculture, College of Animal Science and Technology, Sichuan Agricultural University, 211# Huimin Road, Chengdu, Sichuan, China.
Department of Aquaculture, College of Animal Science and Technology, Sichuan Agricultural University, 211# Huimin Road, Chengdu, Sichuan, China; The Key Laboratory of Mariculture, Ministry of Education, Fisheries College, Ocean University of China, 5# Yushan Road, Qingdao, Shandong, China.
出版信息
Comp Biochem Physiol A Mol Integr Physiol. 2019 Aug;234:106-113. doi: 10.1016/j.cbpa.2019.04.022. Epub 2019 Apr 30.
Urocortin-3 (UCN3) as a brain-gut peptide inhibits food intake of animal, but the underlying mechanism is not clear. To explore the appetite mechanism about the action of UCN3 in fish, intraperitoneal injection of UCN3 with CCK8, Lorglumide (CCK1R antagonist) or LY225910 (CCK2R antagonist) were conducted. Siberian sturgeon administrated with UCN3 and CCK8 showed a drastic reduction in food intake. The anorectic effect of UCN3 was significantly blocked by LY225910, but not affected by Lorglumide. Furthermore, LY225910 could effectively reverse appetite factor mRNA expressions, including cck, pyy, cart, npy, ucn3, apelin and nucb2 in the whole brain, stomach and intestinum valvula, but Lorglumide could only partially reverse these effects, suggesting the anorectic effect of UCN3 may be primarily mediated CCK2R in Siberian sturgeon. This study indicates for the first time in fish that UCN3 may inhibit food intake in coordination with CCK and CCK2R.
尿皮质素3（UCN3）作为一种脑肠肽可抑制动物的食物摄取，但其潜在机制尚不清楚。为探究UCN3在鱼类中发挥作用的食欲机制，进行了腹腔注射UCN3与胆囊收缩素8（CCK8）、洛谷胺（CCK1R拮抗剂）或LY225910（CCK2R拮抗剂）的实验。注射UCN3和CCK8的西伯利亚鲟鱼食物摄取量大幅减少。LY225910可显著阻断UCN3的厌食作用，但洛谷胺对其无影响。此外，LY225910可有效逆转全脑、胃和肠瓣中食欲因子mRNA的表达，包括cck、pyy、cart、npy、ucn3、apelin和nucb2，但洛谷胺只能部分逆转这些作用，这表明UCN3的厌食作用在西伯利亚鲟鱼中可能主要由CCK2R介导。本研究首次在鱼类中表明，UCN3可能与CCK和CCK2R协同抑制食物摄取。
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