Zhang Xin, Tang Ni, Qi Jinwen, Wang Shuyao, Hao Jin, Wu Yuanbing, Chen Hu, Tian Zhengzhi, Wang Bin, Chen Defang, Li Zhiqiong
Department of Aquaculture, College of Animal Science and Technology, Sichuan Agricultural University, 211# Huimin Road, Chengdu, China.
Sci Rep. 2017 Sep 29;7(1):12413. doi: 10.1038/s41598-017-12646-3.
To explore the effect of CCK on food intake in Siberian sturgeon, cck cDNA sequence of 1005 bp was obtained, and cck mRNA possessed the highest expression in brain. The expressions of cck were significantly increased after feeding 1 and 3 h, while displaying significant decrease after fasting within 15 days in brain and duodenum. Re-feeding for 3 days induced cck level returned to basic level. Acute i.p. injection experiment showed 100 and 200 ng/g BW CCK8 inhibited the food intake in 0-1 h together with the cumulative food intake within 3 h. 7 days chronic i.p. injection of 100 and 200 ng/g BW CCK8, both daily food intake and cumulative food intake were significantly decreased. In addition, chronic i.p injection of CCK8 induced the expression of feeding related factors changes including cck, ucn3, cart, apelin, pyy and npy in respective organization. Moreover, as revealed by the results, Lorglumide, the CCK1R selective antagonist, effectively reversed the inhibitory effects of CCK8 on food intake and the levels of feeding related factors. On the other hand, LY 225910, the CCK2R selective antagonist, partially reversed these effects. These results indicate CCK is a satiety factor inhibits the feeding of Siberian sturgeon primarily through CCK1R.
为探究胆囊收缩素（CCK）对西伯利亚鲟摄食的影响，获得了1005bp的cck cDNA序列，且cck mRNA在脑中表达量最高。喂食1小时和3小时后，脑中cck的表达显著增加，而禁食15天内，脑和十二指肠中cck的表达显著下降。重新喂食3天可使cck水平恢复至基础水平。急性腹腔注射实验表明，100和200 ng/g体重的CCK8在0 - 1小时内抑制摄食，并抑制3小时内的累积摄食量。连续7天腹腔注射100和200 ng/g体重的CCK8，每日摄食量和累积摄食量均显著下降。此外，慢性腹腔注射CCK8可诱导各组织中与摄食相关因子的表达发生变化，包括cck、ucn3、cart、apelin、pyy和npy。而且，结果显示，CCK1R选择性拮抗剂洛谷胺可有效逆转CCK8对摄食及摄食相关因子水平的抑制作用。另一方面，CCK2R选择性拮抗剂LY 225910可部分逆转这些作用。这些结果表明，CCK是一种饱足因子，主要通过CCK1R抑制西伯利亚鲟的摄食。
