Berry Elaine D, Hutkins Robert W, Mandigo Roger W
Department of Food Science and Technology, University of Nebraska-Lincoln, Lincoln, Nebraska 68583-0919 and Department of Animal Science, University of Nebraska-Lincoln, Nebraska 68583-0908.
J Food Prot. 1991 Sep;54(9):681-686. doi: 10.4315/0362-028X-54.9.681.
The ability of a bacteriocin-producing Pediococcus acidilactici to control postprocessing Listeria monocytogenes contamination of frankfurters was examined. Bacteriocin-producing P. acidilactici JD1-23 or its plasmid-cured derivative JD-M and a five-strain composite of L. monocytogenes were inoculated onto fully processed frankfurters. Under normal storage conditions at 4°C under vacuum, L. monocytogenes without added pediococci grew from an initial level of 10 CFU/g to a final level of 10 CFU/g over 60 d, with a lag time of 20-30 d. Under the same conditions, high levels (10 CFU/g) of either pediococcal strain inhibited growth of L. monocytogenes up to 60 d, although no reduction of cells occurred. With low levels of pediococci (10-10 CFU/g), Listeria grew, although the lag time was increased, and a bacteriocin effect was observed on frankfurters inoculated with JD1-23. In additional experiments done at 4°C and 15°C under aerobic and anaerobic conditions, levels of 10-10 CFU/g of either pediococci were observed to control Listeria growth on frankfurters at 15°C under anaerobic conditions for up to 15 d. At 4°C under aerobic conditions, L. monocytogenes grew on frankfurters inoculated with JD-M, while JD1-23 inhibited growth of the organism up to 30 d. At 15°C under aerobic conditions, L. monocytogenes grew in the presence of either pediococci, although a bacteriocin effect was indicated. Package atmosphere was observed to affect L. monocytogenes growth on this product.
研究了产细菌素的嗜酸乳杆菌对法兰克福香肠后加工过程中单核细胞增生李斯特菌污染的控制能力。将产细菌素的嗜酸乳杆菌JD1 - 23或其质粒消除衍生物JD - M与单核细胞增生李斯特菌的五菌株复合物接种到完全加工好的法兰克福香肠上。在4°C真空正常储存条件下，未添加嗜酸乳杆菌的单核细胞增生李斯特菌从初始水平10 CFU/g在60天内增长到最终水平10 CFU/g，延迟期为20 - 30天。在相同条件下，两种嗜酸乳杆菌菌株的高浓度（10 CFU/g）在长达60天内抑制了单核细胞增生李斯特菌的生长，尽管细胞数量没有减少。嗜酸乳杆菌低浓度（10 - 10 CFU/g）时，李斯特菌能够生长，尽管延迟期延长，并且在接种JD1 - 23的法兰克福香肠上观察到了细菌素效应。在4°C和15°C有氧和厌氧条件下进行的额外实验中，观察到嗜酸乳杆菌浓度为10 - 10 CFU/g时，在15°C厌氧条件下对法兰克福香肠上的李斯特菌生长控制长达15天。在4°C有氧条件下，单核细胞增生李斯特菌在接种JD - M的法兰克福香肠上生长，而JD1 - 23在长达30天内抑制了该菌的生长。在15°C有氧条件下，单核细胞增生李斯特菌在两种嗜酸乳杆菌存在时都能生长，尽管显示出细菌素效应。观察到包装气氛会影响该产品上单核细胞增生李斯特菌的生长。
