Schmitt Alessandra C, McCormick Ryan, Cohen Cynthia, Siddiqui Momin T
Emory University School of Medicine, Pathology, Atlanta, Georgia.
Emory University School of Medicine, Pathology, Atlanta, Georgia.
J Am Soc Cytopathol. 2014 Nov-Dec;3(6):303-308. doi: 10.1016/j.jasc.2014.06.001. Epub 2014 Jun 28.
DOG1 is a calcium-activated chloride channel protein that may have a potential role in secretory cells of salivary glands and tumors derived from them. Its role in cytologic specimens is not well documented. This study was performed to evaluate its utility in separating acinic cell carcinoma (AciCC) from other closely related differential diagnoses on cytologic samples. In addition, an immunohistochemical panel consisting of DOG1, p63, and S100 protein to assist in the subclassification of these salivary gland neoplasms with oncocytic differentiation was also investigated.
Thirty-one fine-needle aspiration cell blocks (CBs) of oncocytic salivary gland neoplasms (16 Warthin tumors [WTs], 10 AciCCs, 3 mucoepidermoid carcinomas [MECs], and 2 oncocytomas [ONCs]), and 75 salivary gland resections (7 WTs, 27 AciCCs, 36 MECs, 2 high-grade adenocarcinomas, 2 ONCs, 1 papillary cystadenoma) were immunostained for DOG1, p63, and S100.
DOG1 and p63 were very useful in distinguishing AciCC from WT on CB, because 100% of WTs were DOG1-negative and 87.5% were p63-positive, whereas 70% of AciCCs were DOG1-positive and 100% were p63-negative. The resection results correlated with those on CBs: 100% of WTs were DOG1-negative and 86% were p63-positive, whereas 93% of AciCCs were DOG1-positive and 89% were p63-negative. S100 and DOG1 were negative in both WTs and ONCs, with <10% S100 positivity in AciCCs.
DOG1 was very helpful in separating AciCC from WT, MEC, and ONC. In summary, an immunohistochemical panel including DOG1, p63, and S100 can significantly improve the accuracy of diagnosing oncocytic salivary gland neoplasms on CBs.
DOG1是一种钙激活氯离子通道蛋白，可能在唾液腺分泌细胞及其来源的肿瘤中发挥潜在作用。其在细胞学标本中的作用尚无充分文献记载。本研究旨在评估其在细胞学样本中区分腺泡细胞癌（AciCC）与其他密切相关鉴别诊断的效用。此外，还研究了由DOG1、p63和S100蛋白组成的免疫组织化学检测组合，以协助对这些具有嗜酸性细胞分化的唾液腺肿瘤进行亚分类。
对31例嗜酸性细胞唾液腺肿瘤的细针穿刺细胞块（CBs）（16例沃辛瘤[WTs]、10例腺泡细胞癌[AciCCs]、3例黏液表皮样癌[MECs]和2例嗜酸性细胞瘤[ONCs]）以及75例唾液腺切除术标本（7例WTs、27例AciCCs、36例MECs、2例高级别腺癌、2例ONCs、1例乳头状囊腺瘤）进行DOG1、p63和S100免疫染色。
DOG1和p63在CBs上对区分AciCC与WT非常有用，因为100%的WTs为DOG1阴性，87.5%为p63阳性，而70%的AciCCs为DOG1阳性，100%为p63阴性。切除标本的结果与CBs结果相关：100%的WTs为DOG1阴性，86%为p63阳性，而93%的AciCCs为DOG1阳性且89%为p63阴性。WTs和ONCs中S100和DOG1均为阴性，AciCCs中S100阳性率<10%。
DOG1对区分AciCC与WT、MEC和ONC非常有帮助。总之，包括DOG1、p63和S100的免疫组织化学检测组合可显著提高CBs上嗜酸性细胞唾液腺肿瘤诊断的准确性。
