College of Veterinary Medicine and Institute of Veterinary Science, Kangwon National University, Chuncheon, 24341, Republic of Korea.
J Microbiol. 2019 Jun;57(6):423-430. doi: 10.1007/s12275-019-8635-4. Epub 2019 May 27.
Antibiotics have long been used for anti-infective control of bacterial infections, growth promotion in husbandry, and prophylactic protection against plant pathogens. However, their inappropriate use results in the emergence and spread of multiple drug resistance (MDR) especially among various bacterial populations, which limits further administration of conventional antibiotics. Therefore, the demand for novel anti-infective approaches against MDR diseases becomes increasing in recent years. The peptide nucleic acid (PNA)-based technology has been proposed as one of novel anti-infective and/or therapeutic strategies. By definition, PNA is an artificially synthesized nucleic acid mimic structurally similar to DNA or RNA in nature and linked one another via an unnatural pseudo-peptide backbone, rendering to its stability in diverse host conditions. It can bind DNA or RNA strands complimentarily with high affinity and sequence specificity, which induces the target-specific gene silencing by inhibiting transcription and/or translation. Based on these unique properties, PNA has been widely applied for molecular diagnosis as well as considered as a potential anti-infective agent. In this review, we discuss the general features of PNAs and their application to various bacterial pathogens as new anti-infective or antimicrobial agents.
抗生素长期以来一直被用于抗感染控制细菌感染、畜牧业中的生长促进和植物病原体的预防性保护。然而，它们的不当使用导致了多重耐药性（MDR）的出现和传播，特别是在各种细菌群体中，这限制了常规抗生素的进一步使用。因此，近年来人们对针对 MDR 疾病的新型抗感染方法的需求不断增加。基于肽核酸（PNA）的技术已被提议作为一种新型抗感染和/或治疗策略。根据定义，PNA 是一种人工合成的核酸类似物，在结构上与自然界中的 DNA 或 RNA 相似，并通过非天然的假肽骨架彼此连接，使其在各种宿主条件下稳定。它可以与 DNA 或 RNA 链互补结合，具有高亲和力和序列特异性，通过抑制转录和/或翻译来诱导针对特定基因的沉默。基于这些独特的特性，PNA 已被广泛应用于分子诊断，并被认为是一种有潜力的抗感染药物。在这篇综述中，我们讨论了 PNA 的一般特征及其作为新型抗感染或抗菌剂在各种细菌病原体中的应用。
