Department of Applied Chemistry, National Chiao Tung University, Hsinchu 300, Taiwan, Republic of China.
Department of Applied Chemistry, National Chiao Tung University, Hsinchu 300, Taiwan, Republic of China.
Methods. 2019 Sep 15;168:18-23. doi: 10.1016/j.ymeth.2019.04.023. Epub 2019 May 2.
The development of fluorescent probes to detect trace metal ions in biological samples has been in great need. Herein, a fluorescence turn-on sensor (PHC) was designed for highly selective detection of Cu ions. The probe PHC shows weak fluorescence due to imine isomerization. With Cu, a significant blue emission due to Cu-induced oxidation of imine to a carboxylate group is observed. The turn-on process is observed with a 63-fold increase of fluorescence quantum yield (from 0.004 to 0.252). The emission intensity has a good linear relation at Cu concentrations of 0-40 μM. The detection limit is estimated as 8 nM (S/N = 3). The maximum emission change induced by Cu is found in the pH range of 6.5-8.0. The probe PHC can be applied in detecting Cu in living cells monitored by confocal fluorescence microscopy imaging.
荧光探针用于检测生物样品中痕量金属离子的发展非常有必要。本文设计了一种荧光开启传感器（PHC），用于高选择性检测 Cu 离子。由于亚胺的异构化，探针 PHC 显示出微弱的荧光。与 Cu 反应后，由于 Cu 诱导亚胺氧化为羧酸盐基团，观察到显著的蓝色发射。开启过程伴随着荧光量子产率从 0.004 增加到 0.252 的 63 倍增加。在 Cu 浓度为 0-40µM 范围内，发射强度具有良好的线性关系。检测限估计为 8nM（S/N=3）。Cu 引起的最大发射变化发生在 pH 值为 6.5-8.0 的范围内。该探针 PHC 可用于通过共聚焦荧光显微镜成像检测活细胞中的 Cu。
