Swendsen C L, Chilton F H, O'Flaherty J T, Surles J R, Piantadosi C, Waite M, Wykle R L
Biochim Biophys Acta. 1987 May 13;919(1):79-89. doi: 10.1016/0005-2760(87)90220-7.
The incorporation of radiolabeled arachidonic acid and saturated fatty acids into choline-linked phosphoglycerides (PC) of rabbit and human neutrophils was investigated by resolving the individual molecular species by reversed-phase high performance liquid chromatography. PC from neutrophils incubated with a mixture of [3H]arachidonic acid and [14C]stearic or [14C]palmitic acid contains both radiolabels; however, double labeling of individual molecular species is minimal. After labeling for 2 h, the [3H]arachidonate is distributed almost equally between diacyl and 1-O-alkyl-2-acyl species, but it is incorporated into diacyl species containing unlabeled stearate or palmitate at the sn-1 position. In contrast, labeled saturated fatty acids are incorporated only into diacyl species and contain predominantly oleate and linoleate at the sn-2 position. Labeled linoleate is not incorporated into ether-linked species, but is found in the same species as labeled stearate. The findings suggest that mechanisms exist in neutrophils for specific shunting of exogenous arachidonic acid into certain phospholipid molecular species and support the concept that the 1-O-alkyl-2-arachidonoyl species may be a functionally segregated pool of arachidonic acid within the PC of neutrophils.
通过反相高效液相色谱法分离各个分子种类，研究了放射性标记的花生四烯酸和饱和脂肪酸掺入兔和人中性粒细胞的胆碱连接磷脂（PC）中的情况。用[3H]花生四烯酸与[14C]硬脂酸或[14C]棕榈酸的混合物孵育的中性粒细胞中的PC含有两种放射性标记；然而，单个分子种类的双重标记极少。标记2小时后，[3H]花生四烯酸盐几乎均匀地分布在二酰基和1-O-烷基-2-酰基种类之间，但它掺入到在sn-1位置含有未标记硬脂酸盐或棕榈酸盐的二酰基种类中。相比之下，标记的饱和脂肪酸仅掺入二酰基种类中，并且在sn-2位置主要含有油酸酯和亚油酸酯。标记的亚油酸酯不掺入醚连接种类中，但与标记的硬脂酸盐存在于相同的种类中。这些发现表明中性粒细胞中存在将外源性花生四烯酸特异性分流到某些磷脂分子种类中的机制，并支持1-O-烷基-2-花生四烯酰基种类可能是中性粒细胞PC内功能上分离的花生四烯酸池的概念。
