Nikbakht Elham, Jamaluddin Rosita, Redzwan S Mohd, Khalesi Saman
1 Menzies Health Institute Queensland, School of Medical Science, Griffith University, Gold Coast, Australia.
2 Department of Nutrition and Dietetics, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Selangor, Malaysia.
Int J Vitam Nutr Res. 2018 Jun;88(3-4):199-208. doi: 10.1024/0300-9831/a000513. Epub 2019 May 6.
Aflatoxin B(AFB) is a toxic compound commonly found in some crops with an adverse health effect on human and animals. Some beneficial microorganisms (or probiotics) such as lactic acid bacteria have shown the ability to reduce the bioavailability of aflatoxins and its intestinal absorption. However, the dose and duration of aflatoxins exposure and probiotic treatment can influence the ability of probiotics to remove aflatoxins. Therefore, this research aimed to investigate the efficacy of oral probiotic Shirota strain (LcS) induction in an acute exposure to AFB in rats. Experimentally, Sprague Dawley rats were divided into three groups: AFB only (n = 9); AFB treated with LcS (n = 9); and control (no AFB exposure) (n = 6) groups. The blood AFB level of rats treated with LcS was slightly lower than the untreated AFB induced rats (11.12 ± 0.71 vs 10.93 ± 0.69 ng g). Also, LcS treatment slightly moderated the liver and kidney biomarkers in AFB induced rats. However, a trend for a significant difference was only observed in ALT of AFB induced rats treated with LcS compared to their counterparts (126.11 ± 36.90 vs 157.36 ± 15.46, = 0.06). Rats' body weight decreased in all animals force-fed with AFB with no significant difference between LcS treatment compared to the counterpart. In conclusion, this experiment indicated that probiotic LsC was able to slightly ameliorate the adverse effect of an acute exposure to AFB in rats. However, future studies with longer probiotics treatment or higher probiotics dose is required to confirm these findings.
黄曲霉毒素B（AFB）是一种常见于某些作物中的有毒化合物，对人和动物的健康有不良影响。一些有益微生物（或益生菌），如乳酸菌，已显示出降低黄曲霉毒素生物利用度及其肠道吸收的能力。然而，黄曲霉毒素暴露的剂量和持续时间以及益生菌治疗会影响益生菌去除黄曲霉毒素的能力。因此，本研究旨在调查口服益生菌嗜热链球菌（LcS）对大鼠急性暴露于AFB的诱导效果。实验中，将斯普拉格-道利大鼠分为三组：仅AFB组（n = 9）；AFB+LcS治疗组（n = 9）；以及对照组（未暴露于AFB）（n = 6）。接受LcS治疗的大鼠血液中AFB水平略低于未治疗的AFB诱导大鼠（11.12±0.71对10.93±0.69 ng/g）。此外，LcS治疗略微缓解了AFB诱导大鼠的肝脏和肾脏生物标志物。然而，与未治疗的大鼠相比，仅在接受LcS治疗的AFB诱导大鼠的谷丙转氨酶（ALT）中观察到显著差异的趋势（126.11±36.90对157.36±15.46，P = 0.06）。所有强制喂食AFB的动物体重均下降，LcS治疗组与未治疗组之间无显著差异。总之，本实验表明益生菌LcS能够略微改善大鼠急性暴露于AFB的不良影响。然而，需要进一步进行更长时间的益生菌治疗或更高剂量的益生菌研究来证实这些发现。
