Institute of Biochemistry and Microbiology, Faculty of Chemical and Food Technology, Slovak University of Technology, Radlinského 9, 812 37 Bratislava, Slovak Republic.
Can J Microbiol. 2019 Sep;65(9):653-667. doi: 10.1139/cjm-2018-0670. Epub 2019 May 6.
We continued our study of high-molecular-mass proteases (HMMPs) using several strains of the genus , and other filamentous fungi ( and ). We found that five strains secreted HMMPs into the media after induction with bovine serum albumin. and secreted proteases in the absence of inducer, while or did not secrete proteolytic activity (PA). The activity of HMMPs secreted by or intracellularly located in spp. represents the predominant part of cellular PA, according to zymogram patterns. This observation allowed the study of HMMPs' physiological role(s) independent from the secretion. In studying conidiation, we found that illumination significantly stimulated PA in strains. In the IMI 206040 strain, we demonstrated that this stimulation is dependent on the BLR1 and BLR2 receptors. No stimulation of PA was observed when mechanical injury was used as an elicitor of conidiation. Compounds used as inhibitors or activators of conidiation exerted no congruent effects on both PA and conidiation. These results do not favour a direct role of HMMPs in conidiation. Probably, HMMP activity may be involved in the process of the activation of metabolism during vegetative growth, differentiation, and aging-related processes.
我们继续使用几株 和其他丝状真菌（ 和 ）研究高分子质量蛋白酶（HMMPs）。我们发现，在用牛血清白蛋白诱导后，五种 菌株会将 HMMPs 分泌到培养基中。 和 在没有诱导剂的情况下分泌蛋白酶，而 或 则不分泌蛋白水解活性（PA）。根据同工酶图谱， 或 菌株细胞内存在的 HMMPs 的活性代表了细胞 PA 的主要部分。这种观察使我们能够独立于分泌来研究 HMMPs 的生理作用。在研究分生孢子形成时，我们发现光照会显著刺激 菌株中的 PA。在 IMI 206040 菌株中，我们证明这种刺激依赖于 BLR1 和 BLR2 受体。当使用机械损伤作为分生孢子形成的激发剂时，没有观察到 PA 的刺激。用作分生孢子形成抑制剂或激活剂的化合物对 PA 和分生孢子形成均没有一致的影响。这些结果不支持 HMMPs 在分生孢子形成中的直接作用。可能，HMMP 活性可能参与营养生长、分化和与年龄相关的过程中代谢激活的过程。
