用于鉴定抗棘阿米巴药物的病原体盒筛选。
Screening of the pathogen box for the identification of anti-Acanthamoeba agents.
作者信息
Sifaoui Ines, Reyes-Batlle María, López-Arencibia Atteneri, Chiboub Olfa, Bethencourt-Estrella Carlos J, San Nicolás-Hernández Desirée, Rodríguez Expósito Rubén L, Rizo-Liendo Aitor, Piñero José E, Lorenzo-Morales Jacob
机构信息
University Institute of Tropical Diseases and Public Health, Universidad de La Laguna, Avda Francisco Sanchez s/n, Campus de Anchieta, 38271, la Laguna Tenerife, Canary Islands, Spain; Laboratoire Matériaux-Molécules et Applications, IPEST, B.P 51 2070, La Marsa, University of Carthage, Tunisia.
University Institute of Tropical Diseases and Public Health, Universidad de La Laguna, Avda Francisco Sanchez s/n, Campus de Anchieta, 38271, la Laguna Tenerife, Canary Islands, Spain.
出版信息
Exp Parasitol. 2019 Jun;201:90-92. doi: 10.1016/j.exppara.2019.04.013. Epub 2019 May 4.
Acanthamoeba are free living amoeba that have been isolated from different environments like soil, water, air dust. Moreover, they are also able to act as opportunist pathogens, mainly causing a fatal encephalitis and also keratitis in both human and animals. This study was aimed to evaluate the activity of the Medicines for Malaria Venture (MMV) compounds against the trophozoite stage of Acanthamoeba castellanii Neff. Sixteen compounds showed ≥90% inhibition of parasite growth in the initial screen (10 μM). Those set were further evaluated to determine the inhibitor concentration that inhibit the 50% of the initial population and cytotoxicity against murine macrophages. Among the compounds included in the pathogen box, pentamidine and posaconazole were the most effective against this parasite with an of IC of 0.567 ± 0.04 and 0.630 ± 0.11, respectively.
棘阿米巴是自由生活的变形虫，已从土壤、水、空气尘埃等不同环境中分离出来。此外，它们还能作为机会致病菌，主要导致人类和动物患上致命性脑炎以及角膜炎。本研究旨在评估疟疾药物事业（MMV）化合物对卡氏棘阿米巴内夫滋养体阶段的活性。在初始筛选（10μM）中，16种化合物对寄生虫生长的抑制率≥90%。对这些化合物进一步评估，以确定抑制初始虫口数量50%的抑制剂浓度以及对小鼠巨噬细胞的细胞毒性。在病原体盒中的化合物中，喷他脒和泊沙康唑对这种寄生虫最有效，IC50分别为0.567±0.04和0.630±0.11。
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