Tsutsumi I, Toppari J, Campeau J D, diZerega G S
Fertil Steril. 1987 Apr;47(4):689-95. doi: 10.1016/s0015-0282(16)59123-7.
Forty-five-day-old rats received daily injections of follicle regulatory protein (FRP). After 15, 30, 45, and 70 days of therapy, serum was measured for testosterone, androstenedione, estradiol, and follicle-stimulating hormone levels. Testes were evaluated for sperm head counts, plasminogen activator activity, weight, and length of seminiferous epithelial stages. In no case was serum follicle-stimulating hormone concentration reduced in FRP-treated rats. After 75 days of treatment, there was a significant decrease in the number of the sperm head counts. After 60 days of treatment, the length of the dark zone of the tubule was longer than that of control. Pregnancy rates for FRP-treated rats were reduced after 45 and 60 days of treatment. In conclusion, systemic injection of FRP alters seminiferous epithelial function by reducing development of mature sperm.
45日龄的大鼠每日接受卵泡调节蛋白（FRP）注射。在治疗15、30、45和70天后，检测血清中的睾酮、雄烯二酮、雌二醇和促卵泡激素水平。评估睾丸的精子头部计数、纤溶酶原激活物活性、重量以及生精上皮阶段的长度。在接受FRP治疗的大鼠中，血清促卵泡激素浓度在任何情况下均未降低。治疗75天后，精子头部计数显著减少。治疗60天后，曲细精管暗区的长度比对照组更长。在治疗45天和60天后，接受FRP治疗的大鼠的受孕率降低。总之，全身注射FRP通过减少成熟精子的发育来改变生精上皮功能。
