NG-Test Carba 5 检测法直接检测血培养阳性标本中产碳青霉烯酶的简化检测方法。
Simplified Testing Method for Direct Detection of Carbapenemase-Producing Organisms from Positive Blood Cultures Using the NG-Test Carba 5 Assay.
机构信息
Istituto di Microbiologia, Università Cattolica del Sacro Cuore, Rome, Italy.
Dipartimento di Scienze di Laboratorio e Infettivologiche, Fondazione Policlinico Universitario A. Gemelli IRCCS, Rome, Italy.
出版信息
Antimicrob Agents Chemother. 2019 Jun 24;63(7). doi: 10.1128/AAC.00550-19. Print 2019 Jul.
Abstract
We directly tested 484 organisms from clinical ( = 310) and simulated ( = 174) positive blood cultures using the NG-Test Carba 5 assay for carbapenemase-producing detection. The assay identified all but 4 of the KPC (170/171), OXA-48-like (22/22), VIM (19/21), and NDM (14/15) producers with no false positives. Among the clinical organisms tested, 122 of 123 KPC, 1 of 1 OXA-48-like, and 1 of 2 VIM producers were detected by the assay. Some VIM and NDM producers yielded scant but still-readable bands with the assay. No organisms produced the IMPs that the assay was designed to detect.
摘要
我们使用 NG-Test Carba 5 检测试剂盒直接对 484 株来自临床（ = 310）和模拟（ = 174）阳性血培养物的微生物进行了检测，以检测产碳青霉烯酶的情况。该检测试剂盒鉴定出了除 4 株（KPC 的 170/171、OXA-48 样的 22/22、VIM 的 19/21 和 NDM 的 14/15）外的所有产碳青霉烯酶的微生物，没有假阳性。在检测的临床微生物中，123 株 KPC 中有 122 株、1 株 OXA-48 样、2 株 VIM 中有 1 株被该检测试剂盒检测到。一些 VIM 和 NDM 产酶菌的条带虽然稀疏，但仍可读。该检测试剂盒没有检测到设计用来检测的 IMP 产酶菌。
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