RESIST-5 O.O.K.N.V. 和 NG-Test Carba 5 检测法快速检测血培养阳性的产碳青霉烯酶肠杆菌科细菌：一项比较研究。
RESIST-5 O.O.K.N.V. and NG-Test Carba 5 assays for the rapid detection of carbapenemase-producing Enterobacterales from positive blood cultures: a comparative study.
机构信息
Microbiology and Virology Unit, University Hospital Città della Salute e della Scienza di Torino, Turin, Italy.
Microbiology and Virology Unit, University Hospital Città della Salute e della Scienza di Torino, Turin, Italy.
出版信息
J Hosp Infect. 2020 Jun;105(2):162-166. doi: 10.1016/j.jhin.2020.03.022. Epub 2020 Apr 16.
We prospectively compared the performance of RESIST-5 O.O.K.N.V. and NG-Test Carba 5 assays directly from blood cultures spiked with 130 characterized Enterobacterales isolates. Overall, both assays yielded 100% sensitivity to detect KPC-type carbapenemases and OXA-48-like carbapenemases. Both assays failed to detect KPC-31 and KPC-33, D179Y point mutation variants of KPC-3 and KPC-2, that are deprived of carbapenemase activity and confer resistance to ceftazidime-avibactam. On blood culture bacterial pellets, NDM- and VIM-type carbapenemases were detected in 50.0% and 52.2%, respectively, by RESIST-5 O.O.K.N.V. vs 100% by NG-Test Carba 5. The sensitivity of RESIST-5 O.O.K.N.V. improved to 100% and 95.6%, respectively, by performing the assay on 4-h early subculture.
我们前瞻性地比较了 RESIST-5 O.O.K.N.V. 和 NG-Test Carba 5 检测方法在直接从血培养物中检测 130 株特征明确的肠杆菌科分离株的性能。总体而言，两种检测方法对检测 KPC 型碳青霉烯酶和 OXA-48 样碳青霉烯酶的敏感性均为 100%。两种检测方法均无法检测到 KPC-31 和 KPC-33，它们是 KPC-3 和 KPC-2 的 D179Y 点突变变体，缺乏碳青霉烯酶活性并对头孢他啶-阿维巴坦具有耐药性。在血培养物细菌沉淀中，RESIST-5 O.O.K.N.V. 检测到 50.0%的 NDM 型和 52.2%的 VIM 型碳青霉烯酶，而 NG-Test Carba 5 则检测到 100%。通过对 4 小时早期亚培养物进行检测，RESIST-5 O.O.K.N.V. 的敏感性分别提高到 100%和 95.6%。
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