Sandrin M S, Lovering K E, Tachas G, Collins P R, McKenzie I F
Immunogenetics. 1987;25(5):279-83. doi: 10.1007/BF00404419.
Human DNA was transfected into mouse L cells and tk+ HuLy-m2+ (= CD7+) transfectants isolated after growth in hypoxanthine, aminopterin, thymidine medium and repeated cloning. After several cycles of transfection, greater than 90% of HuLy-m2+ L cells could be detected, by rosetting and by cytofluorography, which showed the transfectants to have a density of CD7 two to five times that found on peripheral blood lymphocytes. Despite this, the 37 kd CD7+ dimer could only be identified with difficulty using cell-surface radioiodination and sodium dodecyl sulfate-polyacrylamide gel electrophoresis techniques. An antiserum was produced (C3H anti-HuLy-m2+ L cells) which, after absorption, was shown to react with HuLy-m2+ antigens present on human thymocytes and lymphocytes and on CD7+ transfected L cells.
将人类DNA转染到小鼠L细胞中，在次黄嘌呤、氨基蝶呤、胸腺嘧啶核苷培养基中生长并经过多次克隆后，分离出tk + HuLy - m2 +（= CD7 +）转染子。经过几个转染周期后，通过玫瑰花结试验和细胞荧光成像法可检测到超过90%的HuLy - m2 + L细胞，结果显示转染子的CD7密度是外周血淋巴细胞的两到五倍。尽管如此，使用细胞表面放射性碘化和十二烷基硫酸钠-聚丙烯酰胺凝胶电泳技术，很难鉴定出37kd的CD7 +二聚体。制备了一种抗血清（C3H抗HuLy - m2 + L细胞），经吸收后，该抗血清可与存在于人类胸腺细胞、淋巴细胞以及CD7 +转染L细胞上的HuLy - m2 +抗原发生反应。
