使用长读测序获得 Race 1 和热带 Race 4 分离物的 f. sp. 基因组数据。
Genome Data of f. sp. Race 1 and Tropical Race 4 Isolates Using Long-Read Sequencing.
机构信息
State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Fujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology, Fujian Agriculture and Forestry University, Fuzhou, China.
College of Horticulture, Fujian Agriculture and Forestry University.
出版信息
Mol Plant Microbe Interact. 2019 Oct;32(10):1270-1272. doi: 10.1094/MPMI-03-19-0063-A. Epub 2019 Aug 6.
Fusarium wilt of banana is caused by the soilborne fungal pathogen f. sp. . We generated two chromosome-level assemblies of f. sp. race 1 and tropical race 4 strains using single-molecule real-time sequencing. The f. sp. race 1 and tropical race 4 assemblies had 35 and 29 contigs with contig N50 lengths of 2.08 and 4.28 Mb, respectively. These two new references genomes represent a greater than 100-fold improvement over the contig N50 statistics of the previous short-read-based f. sp. assemblies. The two high-quality assemblies reported here will be a valuable resource for the comparative analysis of f. sp. races at the pathogenic level.
香蕉枯萎病是由土壤传播的真菌病原体尖孢镰刀菌引起的。我们使用单分子实时测序技术生成了尖孢镰刀菌 1 号生理小种和热带 4 号生理小种的两个染色体水平的组装。1 号生理小种和热带 4 号生理小种的组装分别有 35 和 29 个 contigs，contig N50 长度分别为 2.08 和 4.28 Mb。这两个新的参考基因组比以前基于短读长的 1 号生理小种组装的 contig N50 统计数据提高了 100 多倍。这里报道的两个高质量组装将成为在致病性水平上对尖孢镰刀菌生理小种进行比较分析的宝贵资源。
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