Yılmaz U N, Güneş Uysal R F, Dündar Yılmaz B, Tuncer M C
Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, University of Dicle, Diyarbakır, Turkey.
Department of Proshetic Dentistry, Faculty of Dentistry, University of Dicle, Diyarbakır, Turkey.
Folia Morphol (Warsz). 2019;78(4):754-761. doi: 10.5603/FM.a2019.0051. Epub 2019 May 7.
An abscess is a pocket of pus that forms around the root of an infected tooth. In this study, we aimed to investigate the extracellular matrix proteases ADAMTS1, ADAMTS4, osteonectin, and osteopontin expressions in abscess fluid cells in jaws after implantation and prosthesis operation.
In this clinical study, abscess fluids belonging to 17 patients who applied to the Department of Oral and Maxillofacial Surgery were examined histopathologically and immunohistochemically. In the histopathological examination of the abscess fluid, separation of chromatin bridges in the nuclei of neutrophil cells, pyknosis and apoptotic changes in the nucleus, degenerative change in the cytoplasm, and occasional vacuolar structures were observed.
The positive reaction of ADAMTS1 was observed in fibroblast cells, plasma cells, and macrophage cells. The positive reaction of ADAMTS4 was observed in fibroblast cells, osteoclast cells, and some apoptotic leukocyte cells. Osteopontin expression in osteoclastic cells and polymorphonuclear cells was defined as positive. Osteonectin expression was positive in polymorphonuclear leukocytes and hypertrophic fibroblast cells.
ADAMTS1 and ADAMTS4 may induce bone destruction with its distinctive property in alveolar bone resorption, which promotes the activation of osteoclasts, which can accelerate the destruction of the extracellular matrix in the acute phase. Furthermore, osteoclastic activity increased with the increase of osteonectin and osteopontin protein expression due to inflammation in the abscess cases.
脓肿是在受感染牙齿根部周围形成的一个脓腔。在本研究中，我们旨在调查植入和修复手术后颌骨脓肿液细胞中细胞外基质蛋白酶ADAMTS1、ADAMTS4、骨连接蛋白和骨桥蛋白的表达情况。
在这项临床研究中，对17例到口腔颌面外科就诊患者的脓肿液进行了组织病理学和免疫组织化学检查。在脓肿液的组织病理学检查中，观察到中性粒细胞细胞核中染色质桥的分离、细胞核固缩和凋亡变化、细胞质的退行性变化以及偶尔的空泡结构。
在成纤维细胞、浆细胞和巨噬细胞中观察到ADAMTS1的阳性反应。在成纤维细胞、破骨细胞和一些凋亡白细胞中观察到ADAMTS4的阳性反应。破骨细胞和多形核细胞中的骨桥蛋白表达被定义为阳性。骨连接蛋白在多形核白细胞和肥大的成纤维细胞中表达呈阳性。
ADAMTS1和ADAMTS4可能因其在牙槽骨吸收中的独特特性诱导骨破坏，促进破骨细胞的激活，这在急性期可加速细胞外基质的破坏。此外，在脓肿病例中，由于炎症，破骨细胞活性随着骨连接蛋白和骨桥蛋白表达的增加而增强。
