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以果糖作为唯一碳源,在基本培养基中对从巴西生物群落分离出的酵母菌株进行生理学研究,揭示了其潜在的生物技术应用。

Physiology of yeast strains isolated from Brazilian biomes in a minimal medium using fructose as the sole carbon source reveals potential biotechnological applications.

作者信息

de Andrade Silva Cinthia Aparecida, Oka Marta Lígia, Fonseca Gustavo Graciano

机构信息

Laboratory of Bioengineering, Federal University of Grande Dourados, Faculty of Biological and Environmental Sciences, Dourados, MS CEP 79.804-970 Brazil.

出版信息

3 Biotech. 2019 May;9(5):191. doi: 10.1007/s13205-019-1721-9. Epub 2019 Apr 26.

Abstract

The aim of this study was to evaluate the kinetic parameters and the production of metabolites of 13 novel yeasts isolated from a distillery and fruits, and CAT-1, cultivated in fructose-based medium. The yeasts with the highest were obtained from must, BB2, BB1, and BB9 (0.47-0.49 h). CAT-1 (3.02 g gDCM h), BB9 (3.01 g gDCM h), and Recol 41 (2.52 g gDCM h) stood out in terms of . Recol 29, and Recol 03 strains showed the highest (0.30 and 0.28 gDCM g, respectively). Recol 10 and BB9 strains stood out for their higher substrate conversion rates into ethanol (0.44 and 0.41 gEth gS, respectively). Recol 03 presented the poorest performance in substrate consumption (0.87 g gDCM h), while the strains isolated from must and Recol 10 showed the highest ethanol production and the Recol 29 showed the highest biomass conversion.

摘要

本研究的目的是评估从一家酿酒厂和水果中分离出的13种新型酵母以及在基于果糖的培养基中培养的CAT-1的动力学参数和代谢产物的产生。具有最高[具体内容缺失]的酵母来自葡萄汁、BB2、BB1和BB9(0.47 - 0.49小时)。在[具体内容缺失]方面,CAT-1(3.02 ggDCM h)、BB9(3.01 ggDCM h)和Recol 41(2.52 ggDCM h)表现突出。Recol 29和Recol 03菌株显示出最高的[具体内容缺失](分别为0.30和0.28 gDCM g)。Recol 10和BB9菌株因其较高的底物转化为乙醇的速率(分别为0.44和0.41 gEth gS)而突出。Recol 03在底物消耗方面表现最差(0.87 ggDCM h),而从葡萄汁中分离出的菌株和Recol 10显示出最高的乙醇产量,Recol 29显示出最高的生物量转化率。

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